Zhu Luping, Shimada Takashi, Chen Ruoxi, Lu Meiping, Zhang Qingzhao, Lu Wenmin, Yin Min, Enomoto Tadao, Cheng Lei
Department of Otorhinolaryngology, the First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
J Biomed Res. 2012 May;26(3):226-34. doi: 10.7555/JBR.26.20120023. Epub 2012 May 9.
In the current study, we sought to investigate whether lysed Enterococcus faecalis FK-23 (LFK), a heat-killed probiotic preparation, attenuated eosinophil influx into the upper airway and had immunomodulatory activity in a murine allergic rhinitis model. Eighteen BALB/c mice were divided into three groups; the ovalbumin (OVA)-sensitized/challenged group, which received saline orally for 6 weeks (OVA group), the OVA-sensitized/challenged group, which received LFK orally for 6 weeks (LFK-fed group), and the non-sensitized group, which received saline for 6 weeks (saline control group). Nasal rubbing and sneezing were monitored during the study. After the final challenge, interleukin (IL)-4, interferon (IFN)-γ, and OVA-specific IgE levels in the sera and splenocyte culture supernatants were determined, eosinophilic infiltrate into the upper airway was quantified, and splenic CD4+CD25+ regulatory T cells (Tregs) were examined by flow cytometry. We found that nasal rubbing was significantly reduced in LFK-fed mice compared to the OVA group on d 27 and 35, and sneezing was significantly inhibited by LFK administration for 35 d. LFK-fed mice had significantly less eosinophil influx into the nasal mucosa than the OVA group. There were no significant differences between the LFK-fed group and OVA group in the serum and splenocyte culture supernatant levels of IL-4, IFN-γ, and OVA-specific IgE. Interestingly, the LFK-fed mice had a significantly greater percentage of splenic CD4+CD25+ Tregs than OVA group. Our results indicate that oral administration of LFK may alleviate nasal symptoms, reduce nasal eosinophilia, and increase the percentage of CD4+CD25+ Tregs in experimental allergic rhinitis.
在本研究中，我们试图探究溶壁粪肠球菌FK - 23（LFK），一种热灭活益生菌制剂，是否能减轻嗜酸性粒细胞向小鼠上呼吸道的浸润，并在小鼠变应性鼻炎模型中具有免疫调节活性。18只BALB/c小鼠被分为三组：卵清蛋白（OVA）致敏/激发组，口服生理盐水6周（OVA组）；OVA致敏/激发组，口服LFK 6周（LFK喂养组）；未致敏组，口服生理盐水6周（生理盐水对照组）。在研究过程中监测鼻擦和打喷嚏情况。末次激发后，测定血清和脾细胞培养上清液中白细胞介素（IL）-4、干扰素（IFN）-γ和OVA特异性IgE水平，对嗜酸性粒细胞在上呼吸道的浸润进行定量，并通过流式细胞术检测脾脏CD4 + CD25 +调节性T细胞（Tregs）。我们发现，与OVA组相比，LFK喂养组小鼠在第27天和第35天时鼻擦显著减少，LFK给药35天可显著抑制打喷嚏。LFK喂养组小鼠鼻黏膜中的嗜酸性粒细胞浸润明显少于OVA组。LFK喂养组和OVA组在血清和脾细胞培养上清液中IL - 4、IFN -γ和OVA特异性IgE水平方面无显著差异。有趣的是，LFK喂养组小鼠脾脏CD4 + CD25 + Tregs的百分比显著高于OVA组。我们的结果表明，口服LFK可能减轻实验性变应性鼻炎的鼻部症状，减少鼻嗜酸性粒细胞增多，并增加CD4 + CD25 + Tregs的百分比。
