Istanbul University Istanbul Medical Faculty, Departments of Physiology, Istanbul, Turkey.
Clin Exp Rheumatol. 2013 May-Jun;31(3 Suppl 77):25-7. Epub 2013 Apr 3.
Oligoclonal bands (OCB) of immunoglobulins (IgG) in the cerebrospinal fluid (CSF) provides an evidence for the humoral response and have been screened in the CSF and serum of patients revealing 5 different patterns. In this study, patients with Behçet's disease (BD) are screened in a larger sample to potentially provide information about the possible role of CSF oligoclonal immunoglobulins in the diagnosis of this disease.
Paired CSF and serum samples from 121 consecutive BD patients with neurological complaints (43 women and 78 men) were included in this study. Parenchymal NBD was diagnosed in 74 patients, and 22 patients had cerebral venous sinus thrombosis (CVST); of the remaining patients, 18 had primary headache disorders not directly associated with BD, and 7 had a cerebrovascular event. OCB of IgG were detected by isoelectric focusing on agarose and immunoblotting of matched serum and CSF sample pairs. Intrathecal production of IgG only is considered positive (Pattern 2 or 3).
In the whole group, only 8 patients had OCB in the CSF showing pattern 2. All these positive cases had parenchymal neuro-BD (10.8% positive and 78.4% negative in parenchymal neuro-BD group). All other groups were negative.
The rare presence of oligoclonal IgG bands in CSF can be utilized as another laboratory finding in the diagnosis of NBD.
脑脊液（CSF）中免疫球蛋白（IgG）的寡克隆带（OCB）提供了体液反应的证据，并已在有神经症状的患者的 CSF 和血清中进行了筛查，揭示了 5 种不同的模式。在这项研究中，对更大样本量的贝赫切特病（BD）患者进行了筛查，以期为 CSF 寡克隆免疫球蛋白在该疾病诊断中的可能作用提供信息。
本研究纳入了 121 例连续的有神经症状的 BD 患者（43 名女性和 78 名男性）的配对 CSF 和血清样本。74 例患者诊断为实质型 NBD，22 例患者发生脑静脉窦血栓形成（CVST）；其余患者中，18 例为与 BD 无直接关联的原发性头痛障碍，7 例为脑血管事件。通过琼脂糖等电聚焦和配对 CSF 和血清样本的免疫印迹检测 IgG 的 OCB。仅考虑鞘内产生 IgG 为阳性（模式 2 或 3）。
在整个组中，只有 8 例患者的 CSF 中出现 OCB 模式 2。所有这些阳性病例均有实质型神经 BD（实质型神经 BD 组阳性率为 10.8%，阴性率为 78.4%）。其他组均为阴性。
CSF 中寡克隆 IgG 带的罕见存在可作为 NBD 诊断的另一实验室发现。
