Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
Nat Protoc. 2013 May;8(5):836-48. doi: 10.1038/nprot.2013.043. Epub 2013 Apr 4.
Most cases of prostate cancer are now diagnosed as moderate-grade localized disease. These tumor specimens are important tools in the discovery and translation of prostate cancer research; however, unlike more advanced tumors, they are notoriously difficult to grow in the laboratory. We developed a system for efficiently xenografting localized human prostate cancer tissue, and we adapted this protocol to study the interactions between the specific subsets of epithelial and stromal cells. Fresh prostate tissues or isolated epithelial cells are recombined with mouse seminal vesicle mesenchyme (SVM) and grafted under the renal capsule of immunodeficient mice for optimum growth and survival. Alternatively, mouse mesenchyme can be replaced with human prostate fibroblasts in order to determine their contribution to tumor progression. Grafts can be grown for several months to determine the effectiveness of novel therapeutic compounds when administered to host mice, thereby paving the way for personalizing the treatment of individual prostate cancers.
大多数前列腺癌病例现在被诊断为中度局限性疾病。这些肿瘤标本是发现和转化前列腺癌研究的重要工具；然而，与更高级别的肿瘤不同，它们在实验室中生长非常困难。我们开发了一种有效异种移植局部人前列腺癌组织的系统，并对该方案进行了调整，以研究上皮细胞和基质细胞的特定亚群之间的相互作用。将新鲜的前列腺组织或分离的上皮细胞与小鼠精囊间充质（SVM）重组，并在免疫缺陷小鼠的肾包膜下移植，以获得最佳的生长和存活。或者，可以用人前列腺成纤维细胞代替小鼠间充质，以确定它们对肿瘤进展的贡献。可以将移植物培养几个月，以确定当给予宿主小鼠时新型治疗化合物的有效性，从而为个体化治疗个别前列腺癌铺平道路。
