Zemianek Jill M, Lee Sangmook, Guaraldi Mary, Shea Thomas B
Center for Cellular Neurobiology & Neurodegeneration Research, University of Massachusetts Lowell, Lowell, MA 01854, USA.
Int J Dev Neurosci. 2013 Aug;31(5):308-10. doi: 10.1016/j.ijdevneu.2013.03.011. Epub 2013 Apr 3.
A number of laboratories have modeled aspects of synaptic plasticity using neuronal networks established on micro-electrode arrays. Such studies demonstrate that external stimulation can increase or hasten maturation of network signaling as evidenced an increase in complex bursts. Herein, we demonstrate that repetitive stimulation with a recorded synaptic signal was capable of increasing overall signaling, including the percentage of bursts, over a 5-day period, but that this increase was completely prevented by the presence of the GABAergic antagonist bicuculline. These findings demonstrate a critical role for inhibitory neurons in signal maturation following stimulation, which supports the purported role for inhibitory neuronal activity in long-term potentiation and learning in situ.
许多实验室利用在微电极阵列上建立的神经网络对突触可塑性的各个方面进行了建模。此类研究表明，外部刺激可增加或加速网络信号的成熟，复杂爆发的增加即为明证。在此，我们证明，在5天的时间里，用记录的突触信号进行重复刺激能够增加包括爆发百分比在内的整体信号，但这种增加被GABA能拮抗剂荷包牡丹碱完全阻断。这些发现证明了抑制性神经元在刺激后信号成熟中的关键作用，这支持了抑制性神经元活动在长期增强和原位学习中所声称的作用。
