Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo 25280, Coahuila, México.
J Basic Microbiol. 2014 Jan;54(1):28-34. doi: 10.1002/jobm.201200278. Epub 2013 Apr 8.
Ellagitannins (ETs) are phytochemicals derived from secondary metabolism associated to defense system, with complex chemical structures, which have high participation during all stages of protection against microbial infection. In this study, we report the fungal biodegradation of a bioactive ET, named punicaline which was recovered and purified from pomegranate peels and used as carbon source in solid-state culture (SSC) using polyurethane as solid support. SSC was kinetically monitored during 36 h of incubation time. ETs and glycosides consumption were spectrophotometrically determined. Ellagic acid (EA) accumulation was analyzed by HPLC. Several enzymatic activities were assayed (cellulase, xylanase, β-glucosydase, polyphenoloxidase, tannase, and ET hydrolyzing activities). The consumption levels of ETs and glycosides were 66 and 40%, while EA accumulation reached 42.02 mg g(-1). A differential pattern of enzymatic activities was found; evidence from our studies suggests that the ET hydrolyzing activity is directly associated to EA accumulation, and production of this enzyme may represent the most critical step to successfully develop a bioprocess for production of an important bioactive compound, the EA.
鞣花单宁（ETs）是一种次生代谢产物，与防御系统有关，具有复杂的化学结构，在抵御微生物感染的所有阶段都有很高的参与度。在这项研究中，我们报告了一种生物活性 ET（命名为 punicaline）的真菌生物降解，该 ET 是从石榴皮中回收和纯化的，并被用作聚氨酯作为固体支持物的固态培养（SSC）中的碳源。在 36 小时的孵育时间内，对 SSC 进行了动力学监测。通过分光光度法测定 ETs 和糖苷的消耗情况。通过 HPLC 分析鞣酸（EA）的积累。测定了几种酶活性（纤维素酶、木聚糖酶、β-葡萄糖苷酶、多酚氧化酶、单宁酶和 ET 水解活性）。ETs 和糖苷的消耗水平分别为 66%和 40%，而 EA 积累达到 42.02mg/g。发现了一种不同的酶活性模式；我们的研究表明，ET 水解活性与 EA 积累直接相关，这种酶的产生可能代表成功开发生产重要生物活性化合物 EA 的生物工艺的最关键步骤。
