Sports Physiology Department, Centre d'Alt Rendiment (CAR-GIRSANE), Av, Alcalde Barnils 3-5, Sant Cugat del Vallés, Barcelona, 08174, Spain.
J Int Soc Sports Nutr. 2013 Apr 8;10(1):20. doi: 10.1186/1550-2783-10-20.
Strenuous exercise has been classically associated to immune-suppression and consequently to an increased risk of infections, especially at the upper respiratory tract. The administration of dietary nucleotides has been demonstrated useful to maintain the immune function in situations of stress and thus could be an appropriate strategy to counteract the decline of the immune function associated to strenuous exercise. The aim of the present study was to asses the impact of a specific nucleotide formulation (Inmunactive®) on the markers of immune function of athletes after a heavy exercise bout under cold conditions.
Twenty elite male taekwondo athletes were randomly divided into two groups of 10 subjects that were supplemented with placebo (P) or Inmunactive (I) at 480 mg/day during 30 days. At baseline (day 0) and after 4 wk of supplementation (day 30) each subject undertook an exhaustion exercise test using a cycloergometer. Skin temperature, core temperature, heart rate, lactate concentration and rating of perceived exertion (RPE) were recorded during the test. Blood and saliva samples were obtained before and after each exercise test for determination of blood cell concentrations, PHA-stimulated lymphocyte proliferation (PHA-LP) and salivary immunoglobulin A (SIgA).
Exercise tests induced neutrophilia and reduction in lymphocyte blood counts on day 0 and on day 30 in both groups. However, the I group exhibited a faster recovery from the lymphopenic response than the P group, so that lymphocyte levels were higher after 150 min (P < 0.0028). Furthermore, the lymphoproliferative response was modulated by nucleotide supplementation, since it was higher in the I group on day 30 despite an almost significant (P < 0.06) exercise-evoked decrease at baseline.
These findings suggest that supplementation with a nucleotide-based product for 4 weeks could counteract the impairment of immune function after heavy exercise.
剧烈运动通常与免疫抑制有关，因此会增加感染的风险，尤其是在上呼吸道。已经证明，膳食核苷酸的使用有助于在应激情况下维持免疫功能，因此它可能是一种对抗与剧烈运动相关的免疫功能下降的合适策略。本研究的目的是评估特定核苷酸配方（Inmunactive®）对在寒冷条件下进行剧烈运动后运动员免疫功能标志物的影响。
20 名男性跆拳道精英运动员被随机分为两组，每组 10 名运动员，分别在 30 天内每天补充安慰剂（P）或 Inmunactive（I）480mg。在基线（第 0 天）和补充 4 周后（第 30 天），每位受试者都使用旋转式踏车进行了一项耗竭运动测试。在测试过程中记录皮肤温度、核心温度、心率、乳酸浓度和感知努力程度（RPE）。在每次运动测试前后采集血液和唾液样本，用于测定血液细胞浓度、PHA 刺激的淋巴细胞增殖（PHA-LP）和唾液免疫球蛋白 A（SIgA）。
运动测试在第 0 天和第 30 天都会引起中性粒细胞增多和淋巴细胞血液计数减少，在两组中均如此。然而，与 P 组相比，I 组从淋巴细胞减少反应中恢复得更快，因此在 150 分钟后淋巴细胞水平更高（P<0.0028）。此外，核苷酸补充调节了淋巴细胞增殖反应，因为尽管在基线时有一个几乎显著的（P<0.06）运动引起的下降，但在第 30 天 I 组的反应更高。
这些发现表明，补充核苷酸产品 4 周可以对抗剧烈运动后免疫功能的损害。
