Research Centre for Biomedical Engineering, Universitat Politècnica de Catalunya, Jordi Girona, 1-3, 08034, Barcelona, Spain.
Laboratory of Experimental Immunology, Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Barcelona, Catalonia, Spain.
J Int Soc Sports Nutr. 2019 Mar 25;16(1):14. doi: 10.1186/s12970-019-0281-z.
Extreme exercise may alter the innate immune system. Glycans are involved in several biological processes including immune system regulation. However, limited data regarding the impact of glycan supplementation on immunological parameters after strenuous exercise are available. We aimed to determine the impact of a standardized polysaccharide-based multi-ingredient supplement, Advanced Ambrotose© complex powder (AA) on salivary secretory Immunoglobulin A (sIgA) and pro- and anti-inflammatory protein levels before and after a marathon in non-elite runners.
Forty-one male marathon runners who completed the 42.195 km of the 2016 Barcelona marathon were randomly assigned to two study groups. Of them, n = 20 (48%) received the AA supplement for 15 days prior the race (AA group) and n = 21 (52%) did not receive any AA supplement (non-AA group). Saliva and blood samples were collected the day before the marathon and two days after the end of the race. Salivary IgA, pro-inflammatory chemokines (Gro-alpha, Gro-beta, MCP-1) and anti-inflammatory proteins (Angiogenin, ACRP, Siglec 5) were determined using commercially ELISA kits in saliva supernatant. Biochemical parameters, including C-reactive protein, cardiac biomarkers, and blood hemogram were also evaluated.
Marathon runners who did not receive the AA supplement experienced a decrease of salivary sIgA and pro-inflammatory chemokines (Gro-alpha and Gro-beta) after the race, while runners with AA supplementation showed lower levels of anti-inflammatory chemokines (Angiogenin). Gro-alpha and Gro-beta salivary levels were lower before the race in the AA group and correlated with blood leukocytes and platelets.
Changes in salivary sIgA and inflammatory chemokines, especially Gro-alfa and Gro-beta, were observed in marathon runners supplemented with AA prior to the race. These findings suggested that AA may have a positive effect on immune response after a strenuous exercise.
剧烈运动可能会改变先天免疫系统。糖链参与包括免疫系统调节在内的多种生物学过程。然而，关于剧烈运动后糖链补充对免疫参数的影响的数据有限。我们旨在确定一种标准化的基于多糖的多成分补充剂——高级 Ambrotose©复合粉（AA）对非精英马拉松运动员赛前和赛后马拉松比赛后唾液分泌免疫球蛋白 A（sIgA）和促炎及抗炎蛋白水平的影响。
41 名完成 2016 年巴塞罗那马拉松 42.195 公里比赛的男性马拉松跑者被随机分为两组。其中，n=20（48%）在比赛前 15 天接受 AA 补充剂（AA 组），n=21（52%）未接受任何 AA 补充剂（非-AA 组）。比赛前一天和比赛结束后两天采集唾液和血液样本。使用商业 ELISA 试剂盒检测唾液上清液中的唾液 IgA、促炎趋化因子（Gro-α、Gro-β、MCP-1）和抗炎蛋白（血管生成素、ACRP、Siglec 5）。还评估了生化参数，包括 C 反应蛋白、心脏生物标志物和血液血常规。
未接受 AA 补充剂的马拉松跑者在赛后唾液 sIgA 和促炎趋化因子（Gro-α和 Gro-β）水平下降，而接受 AA 补充剂的跑者抗炎趋化因子（血管生成素）水平较低。AA 组比赛前唾液 Gro-α和 Gro-β 水平较低，与血液白细胞和血小板相关。
在比赛前补充 AA 的马拉松运动员中观察到唾液 sIgA 和炎症趋化因子，特别是 Gro-alfa 和 Gro-beta 的变化。这些发现表明 AA 可能对剧烈运动后的免疫反应产生积极影响。
