Dayalan S Anto Jeya, Darwin Pramod, Prakash S
Department of Biotechnology, Udaya School of Engineering, Ammandivilai Post-629204, Kanyakumari District, Tamil Nadu, India.
Asian Pac J Trop Biomed. 2011 Jan;1(1):15-9. doi: 10.1016/S2221-1691(11)60061-0.
To explore various unexplored locations where Penicillium spp. would be available and study the production of penicillin from the isolated Penicillium spp. in different media with altered carbohydrate source.
The collected soil samples were screened for the isolation of Penicillium chrysogenum (P. chrysogenum) by soil dilution plate. The isolated Penicillium species were further grown in different production media with changes in the carbohydrate source. The extracted penicillin from various isolates was analyzed by HPLC for the efficacy of the product. Further the products were screened with various bacterial species including methicillin resistant Staphylococcus aureus (MRSA). And the work was extended to find the possible action on MRSA, along with characterization using other pathogens.
From the various soil and citrus samples used for analysis, only the soil sample from Government General Hospital of Bangalore, India, and Sanjay Gandhi Hospital, Bangalore, India, showed some potential growth of the desired fungi P. chrysogenum. Different production media showed varied range of growth of Penicillium. Optimum production of penicillin was obtained in maltose which proved maximum zone of inhibition during assay. Characterization of penicillin on pathogens, like wild Escherichia coli strain, Klebsiella spp., and MRSA, gave quite interesting results such as no activity on the later strain as it is resistant. HPLC data provided the analytical and confirmation details of the penicillin produced. Accordingly, the penicillin produced from the soil sample of Government General Hospital had the high milli absorbance unit of 441.5 mAu compared with that of the penicillin produced from Sanjay Gandhi Hospital sample, 85.52 mAu. Therefore, there was a considerable change in quantity of the penicillin produced from both the samples.
The Penicillium spp. could be possibly rich in hospital contaminants and its environments. This research focuses on various unexplored sources of medical ailments, and also shows that the growth of penicillin is high in maltose rich media that could possibly enhance the growth.
探索各种尚未开发的可能存在青霉菌的地点，并研究从分离出的青霉菌在不同碳水化合物源的培养基中生产青霉素的情况。
通过土壤稀释平板法对采集的土壤样本进行筛选，以分离产黄青霉（P. chrysogenum）。将分离出的青霉菌种在不同的生产培养基中培养，改变碳水化合物源。通过高效液相色谱法（HPLC）分析从各种分离物中提取的青霉素，以评估产物的效力。此外，用包括耐甲氧西林金黄色葡萄球菌（MRSA）在内的各种细菌对产物进行筛选。并且将研究扩展到寻找对MRSA的可能作用，以及使用其他病原体进行特性鉴定。
在用于分析的各种土壤和柑橘样本中，只有来自印度班加罗尔政府总医院和印度班加罗尔桑贾伊·甘地医院的土壤样本显示出所需真菌产黄青霉有一定的生长潜力。不同的生产培养基显示出青霉菌不同的生长范围。在麦芽糖中获得了青霉素的最佳产量，在测定中证明其抑菌圈最大。对青霉素在病原体上进行特性鉴定，如野生大肠杆菌菌株、克雷伯菌属和MRSA，得到了相当有趣的结果，例如对后一种菌株没有活性，因为它具有抗性。HPLC数据提供了所生产青霉素的分析和确认细节。因此，与桑贾伊·甘地医院样本所产青霉素的85.52 mAu相比，政府总医院土壤样本所产青霉素的毫吸收单位较高，为441.5 mAu。因此，两个样本所产青霉素的量有相当大的差异。
青霉菌可能在医院污染物及其环境中大量存在。本研究关注各种未被探索的医疗疾病来源，并且还表明在富含麦芽糖的培养基中青霉菌的生长很高，这可能会促进其生长。
