唇形科植物对氯化镉诱导的DNA损伤的保护作用。
Protection of cadmium chloride induced DNA damage by Lamiaceae plants.
作者信息
Thirugnanasampandan Ramaraj, Jayakumar Rajarajeswaran
机构信息
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, GN Mills, Coimbatore-029, Tamil nadu, India.
出版信息
Asian Pac J Trop Biomed. 2011 Oct;1(5):391-4. doi: 10.1016/S2221-1691(11)60086-5.
OBJECTIVE
To analyze the total phenolic content, DNA protecting and radical scavenging activity of ethanolic leaf extracts of three Lamiaceae plants, i.e. Anisomelos malabarica (A. malabarica), Leucas aspera (L. aspera) and Ocimum basilicum (O. basilicum).
METHODS
The total polyphenols and flavonoids were analyzed in the ethanolic leaf extracts of the lamiaceae plants. To determine the DNA protecting activity, various concentrations of the plant extracts were prepared and treated on cultured HepG2 human lung cancer cells. The pretreated cells were exposed to H2O2 to induce DNA damage through oxidative stress. Comet assay was done and the tail length of individual comets was measured. Nitric oxide and superoxide anion scavenging activities of lamiaceae plants were analyzed.
RESULTS
Among the three plant extracts, the highest amount of total phenolic content was found in O. basilicum (189.33 mg/g), whereas A. malabarica showed high levels of flavonoids (10.66 mg/g). O. basilicum also showed high levels of DNA protecting (85%) and radical scavenging activity.
CONCLUSIONS
The results of this study shows that bioactive phenols present in lamiaceae plants may prevent carcinogenesis through scavenging free radicals and inhibiting DNA damage.
目的
分析三种唇形科植物，即马拉巴毛麝香（A. malabarica）、粗糙地笋（L. aspera）和罗勒（O. basilicum）叶片乙醇提取物的总酚含量、DNA保护及自由基清除活性。
方法
对唇形科植物叶片乙醇提取物中的总多酚和黄酮类化合物进行分析。为测定DNA保护活性，制备不同浓度的植物提取物并处理培养的人肝癌HepG2细胞。将预处理后的细胞暴露于过氧化氢，通过氧化应激诱导DNA损伤。进行彗星试验并测量单个彗星的尾长。分析唇形科植物的一氧化氮和超氧阴离子清除活性。
结果
在三种植物提取物中，罗勒的总酚含量最高（189.33毫克/克），而马拉巴毛麝香的黄酮类化合物含量较高（10.66毫克/克）。罗勒还表现出较高的DNA保护（85%）和自由基清除活性。
结论
本研究结果表明，唇形科植物中存在的生物活性酚类物质可能通过清除自由基和抑制DNA损伤来预防癌症发生。
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