实验性诱导阿米巴肝脓肿中溶组织内阿米巴的检测：三种染色方法的比较
Detection of Entamoeba histolytica in experimentally induced amoebic liver abscess: comparison of three staining methods.
作者信息
Ning Tan Zi, Kin Wong Weng, Mustafa Shaymoli, Ahmed Arefuddin, Noordin Rahmah, Cheong Tan Gim, Alfonso Olivos-Garcia, Huat Lim Boon
机构信息
School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
出版信息
Asian Pac J Trop Biomed. 2012 Jan;2(1):61-5. doi: 10.1016/S2221-1691(11)60191-3.
OBJECTIVE
To compare the efficacy of three different tissue stains, namely haematoxylin and eosin (H&E), periodic-acid Schiff (PAS) and immunohistochemical (IHC) stains for detection of Entamoeba histolytica (E. histolytica) trophozoites in abscessed liver tissues of hamster.
METHODS
Amoebic liver abscess was experimentally induced in a hamster by injecting 1 × 10(6) of axenically cultured virulent E. histolytica trophozoites (HM1-IMSS strain) into the portal vein. After a week post-inoculation, the hamster was sacrificed and the liver tissue sections were stained with H&E, PAS and IHC stains to detect the amoebic trophozoite.
RESULTS
The three stains revealed tissue necrosis and amoebic trophozoites, but with varying clarity. H&E and PAS stained the trophozoites pink and magenta, respectively, however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and morphology. On the other hand, IHC stain revealed distinct brown appearance of the trophozoites in the infected liver tissues.
CONCLUSIONS
It can be concluded that out of the three stains, IHC is the best for identification of E. histolytica trophozoites in tissue sections.
目的
比较苏木精和伊红（H&E）、过碘酸希夫（PAS）及免疫组化（IHC）三种不同组织染色法检测仓鼠肝脓肿组织中溶组织内阿米巴滋养体的效果。
方法
通过将1×10(6)无菌培养的强毒溶组织内阿米巴滋养体（HM1-IMSS株）注入门静脉，在仓鼠体内实验性诱导阿米巴肝脓肿。接种一周后，处死仓鼠，将肝组织切片用H&E、PAS及IHC染色法检测阿米巴滋养体。
结果
三种染色法均显示组织坏死及阿米巴滋养体，但清晰度不同。H&E和PAS分别将滋养体染成粉红色和品红色，然而由于其大小和形态相似，难以将染色的滋养体与巨噬细胞区分开来。另一方面，免疫组化染色显示感染肝组织中的滋养体呈现明显的棕色外观。
结论
可以得出结论，在这三种染色法中，免疫组化是组织切片中鉴定溶组织内阿米巴滋养体的最佳方法。
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