旁遮普邦南部大型反刍动物血液样本中牛巴贝斯虫的检测及其对血液学和血清生化指标的影响
Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab.
作者信息
Zulfiqar Samreen, Shahnawaz Sadia, Ali Muhammad, Bhutta Arif Mahmood, Iqbal Shahid, Hayat Sikandar, Qadir Shazia, Latif Muhammad, Kiran Nazia, Saeed Ali, Ali Muhammad, Iqbal Furhan
机构信息
Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan 60800, Pakistan.
出版信息
Asian Pac J Trop Biomed. 2012 Feb;2(2):104-8. doi: 10.1016/S2221-1691(11)60202-5.
OBJECTIVE
To determine the presence of Babesia bovis (B. bovis) in large ruminants in southern Punjab and its effect on hematological and serum biochemical profile of host animals.
METHODS
Blood samples were collected from 144 large ruminants, including 105 cattle and 39 buffaloes, from six districts in southern Punjab including Multan, Layyah, Muzaffar Garh, Bhakar, Bahawalnagar and Vehari. Data on the characteristics of animals and herds were collected through questionnaires. Different blood (hemoglobin, glucose) and serum (ALT, AST, LDH, cholesterol) parameters of calves and cattle were measured and compared between parasite positive and negative samples to demonstrate the effect of B. bovis on the blood and serological profile of infected animals.
RESULTS
27 out of 144 animals, from 5 out of 6 sampling districts, produced the 541-bp fragment specific for B. bovis. Age of animals (P=0.02), presence of ticks on animals (P=0.04) and presence of ticks on dogs associated with herds (P=0.5) were among the major risk factors involved in the spread of bovine babesiosis in the study area. ALT concentrations were the only serum biochemical values that significantly varied between parasite positive and negative cattle.
CONCLUSIONS
: This study has reported for the first time the presence of B. bovis in large ruminant and the results can lead to the prevention of babesiosis in the region to increase the livestock output.
目的
确定旁遮普邦南部大型反刍动物中牛巴贝斯虫（B. bovis）的存在情况及其对宿主动物血液学和血清生化指标的影响。
方法
从旁遮普邦南部包括木尔坦、莱雅、穆扎法尔格尔、巴卡尔、巴哈瓦尔布尔和 Vehari 的六个地区的 144 头大型反刍动物中采集血样，其中包括 105 头牛和 39 头水牛。通过问卷调查收集动物和畜群特征的数据。测量并比较犊牛和成年牛不同的血液（血红蛋白、葡萄糖）和血清（谷丙转氨酶、谷草转氨酶、乳酸脱氢酶、胆固醇）参数在寄生虫阳性和阴性样本之间的差异，以证明牛巴贝斯虫对感染动物血液和血清学指标的影响。
结果
144 只动物中有 27 只，来自 6 个采样地区中的 5 个地区，产生了牛巴贝斯虫特异的 541 碱基对片段。动物年龄（P = 0.02）、动物体表蜱虫的存在（P = 0.04）以及与畜群相关的犬只体表蜱虫的存在（P = 0.5）是研究区域内牛巴贝斯虫病传播的主要风险因素。谷丙转氨酶浓度是寄生虫阳性和阴性牛之间唯一有显著差异的血清生化值。
结论
本研究首次报道了大型反刍动物中牛巴贝斯虫的存在情况，研究结果有助于该地区预防巴贝斯虫病，提高牲畜产量。
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