Chew Ai Lan, Jessica Jeyanthi James Antony, Sasidharan Sreenivasan
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
Asian Pac J Trop Biomed. 2012 Mar;2(3):176-80. doi: 10.1016/S2221-1691(12)60037-9.
To evaluate antioxidant, antimicrobial and cytotoxic activity of different parts (root, flower, leaf and stem) of Leucas aspera (L. aspera) (Labiatae).
Different parts of L. aspera were extracted with 80% (v/v) methanol. The methanol extracts were subjected to antioxidant, antimicrobial and brine shrimp lethality assay.
All the extracts showed moderate to potent antioxidant activity, among which the root extract demonstrated the strongest antioxidant activity with the IC50 value of 6.552 µg/mL. Methanol extract of root possessed antioxidant activity near the range of vitamin E and thus could be a potential rich source of natural antioxidant. In case of antimicrobial screening, crude extracts of root, flower, leaf and stem showed notable antibacterial activity against tested microorganisms. The root extract showed the highest mean zone of inhibition ranging from 9.0-11.0 mm against tested microorganisms, at a concentration of 100 mg/mL. In the brine shrimp lethality bioassay, it was evident that the methanol root extract did not show significant toxicity. The LC50 value for 12 h and 24 h observation was 2.890 mg/mL and 1.417 mg/mL, respectively.
The present finding suggests that the methanol root extract of L. aspera could be developed as pharmaceutical products.
评价糙苏（唇形科）不同部位（根、花、叶和茎）的抗氧化、抗菌和细胞毒性活性。
用80%（v/v）甲醇提取糙苏的不同部位。对甲醇提取物进行抗氧化、抗菌和卤虫致死率测定。
所有提取物均表现出中度至强效的抗氧化活性，其中根提取物的抗氧化活性最强，IC50值为6.552μg/mL。根的甲醇提取物具有接近维生素E范围的抗氧化活性，因此可能是天然抗氧化剂的潜在丰富来源。在抗菌筛选中，根、花、叶和茎的粗提取物对受试微生物表现出显著的抗菌活性。在浓度为100mg/mL时，根提取物对受试微生物的平均抑菌圈最高，范围为9.0 - 11.0mm。在卤虫致死生物测定中，很明显甲醇根提取物没有显示出显著毒性。12小时和24小时观察的LC50值分别为2.890mg/mL和1.417mg/mL。
目前的研究结果表明，糙苏的甲醇根提取物可开发为药品。
