Karim Md Rezaul, Miletti-Gonzalez Karl E, Aryee Alberta N A, Besong Samuel A
Department of Human Ecology (Food and Nutritional Sciences), Delaware State University, Dover, DE 19901, USA.
Department of Biological Sciences, Delaware State University, Dover, DE 19901, USA.
Antioxidants (Basel). 2025 May 30;14(6):666. doi: 10.3390/antiox14060666.
The phytochemical profile and antioxidant activities of bark, and leaves from Cameroon were investigated in this study. The yields of pure methanolic extraction were 11.9%, 11.1% and 10.8% in bark, and leaves, respectively. The total phenolic content was 189.0 ± 16.93, 163.6 ± 14.73 and 114.6 ± 10.38 mg GAE/g and total flavonoid content was 43.25 ± 6.43, 28.31 ± 4.44, and 19.75 ± 4.03 mg RU/g in bark, and leaves, respectively. The antioxidant activities of the plants were evaluated by DPPH, ABTS and FRAP assays. The IC evaluated in bark, and leaves was 109.5 ± 13.2, 132.1 ± 18.7 and 156.1 ± 21.9 µg/mL for DPPH and 98.1 ± 4.8, 101.3 ± 12.1 and 133.9 ± 16.0 µg /mL for ABTS assay. The FRAP value was 61.1 ± 1.5, 50.5 ± 1.5 and 43.4 ± 2.1 µMFe/g in the same sequence. The functional groups for the corresponding phytochemicals, including alkane, alkene, aliphatic ether, ester, amine, α, β-unsaturated ester, alcohol, phenol, carboxylic acid, and aliphatic ketone, were identified through fourier-transform infrared analysis. The identified and quantified phenolic acids in this study were methyl-4-hydroxybenzoic, caffeic, protocatechuic and p-coumaric acid, identified using high-performance liquid chromatography.
本研究对喀麦隆树皮和树叶的植物化学特征及抗氧化活性进行了研究。树皮、树叶纯甲醇提取物的得率分别为11.9%、11.1%和10.8%。树皮、树叶中总酚含量分别为189.0±16.93、163.6±14.73和114.6±10.38 mg GAE/g，总黄酮含量分别为43.25±6.43、28.31±4.44和19.75±4.03 mg RU/g。通过DPPH、ABTS和FRAP法对植物的抗氧化活性进行了评价。树皮、树叶中DPPH法的IC50分别为109.5±13.2、132.1±18.7和156.1±21.9 μg/mL，ABTS法的IC50分别为98.1±4.8、101.3±12.1和133.9±16.0 μg/mL。FRAP值按相同顺序分别为61.1±1.5、50.5±1.5和43.4±2.1 μMFe/g。通过傅里叶变换红外分析确定了相应植物化学物质的官能团，包括烷烃、烯烃、脂肪醚、酯、胺、α,β-不饱和酯、醇、酚、羧酸和脂肪酮。本研究中鉴定和定量的酚酸为4-羟基苯甲酸甲酯、咖啡酸、原儿茶酸和对香豆酸，采用高效液相色谱法进行鉴定。
