Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
J Am Chem Soc. 2013 Apr 24;135(16):5934-7. doi: 10.1021/ja312210c. Epub 2013 Apr 12.
SSEA-4, a sialyl-glycolipid, has been commonly used as a pluripotent human embryonic stem cell marker, and its expression is correlated with the metastasis of some malignant tumors. However, there is no in-depth functional study related to the receptor and the role of this glycolipid. Here, we report the identification of an SSEA-4-binding protein in a breast cancer cell line, MCF-7. By using affinity capture and glycan microarray techniques, the intracellular FK-506 binding protein 4 (FKBP4) was identified to bind directly to SSEA-4. The biological significance of SSEA-4/FKBP4 interaction was investigated.
SSEA-4 是一种唾液酸糖脂，通常被用作多能人胚胎干细胞的标志物，其表达与一些恶性肿瘤的转移相关。然而，目前还没有关于该糖脂的受体和功能的深入功能研究。在这里，我们报告了在乳腺癌细胞系 MCF-7 中鉴定到 SSEA-4 结合蛋白。通过使用亲和捕获和聚糖微阵列技术，鉴定到细胞内 FK-506 结合蛋白 4（FKBP4）直接与 SSEA-4 结合。研究了 SSEA-4/FKBP4 相互作用的生物学意义。
