Lin Weiming, Modiano Jaime F, Ito Daisuke
Department of Veterinary Clinical Sciences and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
Department of Veterinary Medicine, College of Life Sciences, Longyan University, Longyan 364012, China.
J Vet Sci. 2017 Mar 30;18(1):101-104. doi: 10.4142/jvs.2017.18.1.101.
The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1 and SSEA-1 cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.
在几种犬类癌细胞中测定了阶段特异性胚胎抗原（SSEAs）的表达。流式细胞术显示胶质母细胞瘤、黑色素瘤和乳腺癌细胞中有SSEA-1表达，尽管没有细胞表达SSEA-3或SSEA-4。在淋巴瘤、骨肉瘤或血管肉瘤细胞系中未检测到SSEA-1表达。在培养24至72小时之间观察到相对稳定的SSEA-1表达。培养8天后，分选的SSEA-1阳性和阴性细胞将SSEA-1表达重新建立到与未分选细胞中观察到的水平相当的水平。我们的结果首次证明了SSEA-1在几种犬类癌细胞系中的表达。
