Marshall T A, Roberts R J
Department of Pediatrics and Pharmacology, University of Iowa College of Medicine, Iowa City.
J Am Coll Nutr. 1990 Jun;9(3):190-9. doi: 10.1080/07315724.1990.10720369.
Commercial infant formulas, human milk, and lipid emulsions were analyzed for evidence of naturally occurring lipid peroxidation and for susceptibility to an in vitro oxidative challenge using neonatal rat lung, liver, or intestine homogenates. Peroxidation was assessed by quantitation of TBA reactants, diene conjugates, lipid peroxides, and ethane and pentane hydrocarbons. The peroxidation of commercial formulas and human milk was influenced by the nutrient composition, as PUFA and iron enhanced while vitamin E inhibited one or more of the peroxidation pathways. Formulas and lipid emulsions differed in their response to a biological oxidant challenge. Neither neonatal rat lung nor liver tissue were effective in peroxidizing the formula or human milk in vitro, but both formula and human milk were peroxidized by exposure to neonatal rat intestinal tissue. The lipid emulsion was readily peroxidized by neonatal rat lung, liver, and intestinal tissue. The influence of nutrition on survival in hyperoxia was also studied by exposing newborn rat pups to either air or greater than 95% oxygen in the course of feeding Ringer's lactate, Similac 24 + iron, human milk, or Intralipid 10%. The survival of newborn rat pups exposed to air or greater than 95% oxygen was affected by the type of diet received.
对市售婴儿配方奶粉、母乳和脂质乳剂进行了分析，以寻找天然存在的脂质过氧化证据，并使用新生大鼠肺、肝或肠匀浆评估其对体外氧化应激的易感性。通过定量TBA反应物、二烯共轭物、脂质过氧化物以及乙烷和戊烷烃来评估过氧化作用。市售配方奶粉和母乳的过氧化作用受营养成分影响，因为多不饱和脂肪酸（PUFA）和铁会增强，而维生素E会抑制一种或多种过氧化途径。配方奶粉和脂质乳剂对生物氧化剂应激的反应有所不同。新生大鼠的肺和肝组织在体外均无法有效地使配方奶粉或母乳发生过氧化，但配方奶粉和母乳经新生大鼠肠道组织处理后均会发生过氧化。脂质乳剂很容易被新生大鼠的肺、肝和肠道组织过氧化。通过在给新生大鼠幼崽喂食乳酸林格氏液、添加铁的Similac 24、母乳或10%英脱利匹特的过程中，将它们暴露于空气或高于95%的氧气环境中，还研究了营养对高氧环境下存活率的影响。新生大鼠幼崽暴露于空气或高于95%氧气环境下的存活率受所接受饮食类型的影响。
