κ转基因中体细胞突变的分析
Analysis of somatic mutations in kappa transgenes.
作者信息
Hackett J, Rogerson B J, O'Brien R L, Storb U
机构信息
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
出版信息
J Exp Med. 1990 Jul 1;172(1):131-7. doi: 10.1084/jem.172.1.131.
Abstract
We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nuclear matrix association region, kappa enhancer, and C gene were spared. These data indicate that the rearranged kappa gene contains the necessary sequences for targeting of the mutation process, and suggest that the observed localization of mutations to the V region reflects the inherent specificity of this mutation process.
摘要
我们已经研究了从分泌IgG的杂交瘤中克隆出的三个κ转基因的体细胞突变的性质和定位。所有鉴定出的突变都是单碱基替换。突变定位在可变（V）区及其侧翼序列。在每种情况下，核基质结合区、κ增强子和C基因都未发生突变。这些数据表明，重排的κ基因包含突变过程靶向所需的序列，并表明观察到的突变在V区的定位反映了这种突变过程的固有特异性。
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本文引用的文献
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Somatic mutations of immunoglobulin variable genes are restricted to the rearranged V gene.免疫球蛋白可变基因的体细胞突变仅限于重排的V基因。
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Somatic diversification is required to generate the V kappa genes of MOPC 511 and MOPC 167 myeloma proteins.体细胞多样化是产生MOPC 511和MOPC 167骨髓瘤蛋白的Vκ基因所必需的。
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Somatic mutation of immunoglobulin light-chain variable-region genes.免疫球蛋白轻链可变区基因的体细胞突变
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Nucleotide sequence of a region downstream of the mouse CK immunoglobulin gene.小鼠肌酸激酶免疫球蛋白基因下游区域的核苷酸序列。
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Nucleotide sequence of a chromosomal rearranged lambda 2 immunoglobulin gene of mouse.小鼠染色体重排λ2免疫球蛋白基因的核苷酸序列
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The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes.一段包含小鼠κ免疫球蛋白J区和C区基因的5.5千碱基DNA片段的核苷酸序列。
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