Gorski J, Rollini P, Mach B
Science. 1983 Jun 10;220(4602):1179-81. doi: 10.1126/science.6857243.
An important question concerning the mechanism of somatic mutation of immunoglobulin variable (V) genes is whether it involves all of the numerous V genes in a differentiated B cell, independent of location, or if it is restricted to a particular chromosomal site. Comparison of the sequence of two alleles of a given V gene shows that the mutations are limited to the rearranged V gene, while the same V gene on the other chromosome has not undergone mutation. This indicates that a V gene sequence alone is not sufficient for somatic mutation to take place. The mutation is therefore restricted to the rearranged V gene and consequently does not occur before rearrangement.
关于免疫球蛋白可变（V）基因体细胞突变机制的一个重要问题是，它是否涉及分化的B细胞中所有众多的V基因，而与位置无关，或者它是否局限于特定的染色体位点。对给定V基因的两个等位基因序列进行比较表明，突变仅限于重排的V基因，而另一条染色体上的相同V基因未发生突变。这表明仅V基因序列不足以发生体细胞突变。因此，突变仅限于重排的V基因，因而在重排之前不会发生。
