van Wersch J W, Bank C
Haematological Laboratory, De Wever Hospital, Heerlen, The Netherlands.
J Clin Chem Clin Biochem. 1990 Apr;28(4):233-40. doi: 10.1515/cclm.1990.28.4.233.
We compared the results of the Sysmex NE-8000 with those of the Sysmex E-4000 and the Technicon H-6000. Both for the whole blood cell count and the leukocyte differentiation we obtained intra- and inter-assay coefficients of variation fully comparable with those for other cell counters. The carry-over between samples was negligibly small. The linearity range was broad, which implies a universal application in routine haematological analysis. The numerical results from the Sysmex NE-8000 and the Technicon H-6000 corresponded closely, with exception of the mean corpuscular volume and the haematocrit, whereas these parameters showed excellent agreement between the NE-8000 and the E-4000. In the differentiation of leukocytes, the NE-8000 and H-6000 showed good correlation and conformity for lymphocytes, neutrophils and eosinophils; for monocytes and basophils the conformity was poor. We also found a good correlation between the NE-8000 and E-4000 for lymphocytes and neutrophils, whereas the correlation was less for the middle cell ratio and the sum of monocytes, eosinophils and basophils. Furthermore, we compared Receiver Operating Characteristic curves for the different leukocyte subtypes on the NE-8000, the H-6000 and the E-4000, and investigated the flagging frequency in inpatients, outpatients and blood donors.
我们将Sysmex NE - 8000的检测结果与Sysmex E - 4000以及Technicon H - 6000的检测结果进行了比较。在全血细胞计数和白细胞分化检测方面，我们获得的批内和批间变异系数与其他血细胞计数器的变异系数完全可比。样本之间的残留量极小，可以忽略不计。线性范围很宽，这意味着它在常规血液学分析中具有广泛的适用性。Sysmex NE - 8000和Technicon H - 6000的数值结果除平均红细胞体积和血细胞比容外，其余结果非常接近，而这些参数在NE - 8000和E - 4000之间显示出极好的一致性。在白细胞分化方面，NE - 8000和H - 6000在淋巴细胞、中性粒细胞和嗜酸性粒细胞方面显示出良好的相关性和一致性；在单核细胞和嗜碱性粒细胞方面，一致性较差。我们还发现NE - 8000和E - 4000在淋巴细胞和中性粒细胞方面具有良好的相关性，而在中间细胞比率以及单核细胞、嗜酸性粒细胞和嗜碱性粒细胞总和方面相关性较低。此外，我们比较了NE - 8000、H - 6000和E - 4000上不同白细胞亚型的受试者工作特征曲线，并调查了住院患者、门诊患者和献血者的报警频率。
