Han Cheol-Min, Jang Tae-Sik, Kim Hyoun-Ee, Koh Young-Hag
WCU Hybrid Materials Program, Department of Materials Science and Engineering, Seoul National University, Seoul, 151-744, Korea.
J Biomed Mater Res A. 2014 Mar;102(3):793-800. doi: 10.1002/jbm.a.34748. Epub 2013 Jun 4.
This study evaluated the utility of the creation of a nanoporous TiO2 surface to enhance the in vitro biocompatibility and in vivo osseoconductivity of polyetheretherketone (PEEK) implants by providing favorable sites for the effective immobilization of bone morphogenetic protein-2 (BMP-2). A uniform nanoporous TiO2 layer with a pore diameter of ∼70 nm was successfully created by anodizing a Ti film, which had been deposited onto a PEEK substrate via electron beam (e-beam) evaporation technique. This nanoporous, hydrophilic TiO2 surface enabled the efficient immobilization of BMP-2, resulting in a remarkable enhancement in in vitro biocompatibility that was assessed in terms of cell attachment, proliferation, and differentiation. The in vivo animal tests also confirmed that the nanoporous TiO2 surface immobilized with BMP-2 could significantly enhance the osseoconductivity of PEEK implants. The BMP-immobilized PEEK implant with the nanoporous TiO2 surface showed much higher bone-to-implant contact (BIC) ratio (60%) than the bare PEEK (30%), PEEK with the nanoporous TiO2 surface (50%) and even BMP-immobilized PEEK without the nanoporous TiO2 surface (32%).
本研究评估了通过为骨形态发生蛋白-2（BMP-2）的有效固定提供有利位点，创建纳米多孔二氧化钛表面以增强聚醚醚酮（PEEK）植入物的体外生物相容性和体内骨传导性的效用。通过对经由电子束（e束）蒸发技术沉积在PEEK基底上的钛膜进行阳极氧化，成功创建了孔径约为70 nm的均匀纳米多孔二氧化钛层。这种纳米多孔的亲水性二氧化钛表面能够有效固定BMP-2，从而在细胞附着、增殖和分化方面评估的体外生物相容性得到显著增强。体内动物试验也证实，固定有BMP-2的纳米多孔二氧化钛表面可显著增强PEEK植入物的骨传导性。具有纳米多孔二氧化钛表面且固定有BMP的PEEK植入物显示出比裸露的PEEK（30%）、具有纳米多孔二氧化钛表面的PEEK（50%）甚至没有纳米多孔二氧化钛表面且固定有BMP的PEEK（32%）高得多的骨与植入物接触（BIC）率（60%）。
