Guillot R, Pignot-Paintrand I, Lavaud J, Decambron A, Bourgeois E, Josserand V, Logeart-Avramoglou D, Viguier E, Picart C
CNRS, UMR 5628, LMGP, 3 parvis Louis Néel, F-38016 Grenoble, France; Université de Grenoble Alpes, Grenoble Institute of Technology, 3 parvis Louis Néel, F-38016 Grenoble, France.
Institute Albert Bonniot, INSERM U823, ERL CNRS3148, Grenoble, France.
Acta Biomater. 2016 May;36:310-22. doi: 10.1016/j.actbio.2016.03.010. Epub 2016 Mar 7.
The aim of this study was to evaluate the osseointegration of titanium implants (Ti-6Al-4V, noted here TA6V) and poly(etheretherketone) PEEK implants induced by a BMP-2-delivering surface coating made of polyelectrolyte multilayer films. The in vitro bioactivity of the polyelectrolyte film-coated implants was assessed using the alkaline phosphatase assay. BMP-2-coated TA6V and PEEK implants with a total dose of 9.3μg of BMP-2 were inserted into the femoral condyles of New Zealand white rabbits and compared to uncoated implants. Rabbits were sacrificed 4 and 8weeks after implantation. Histomorphometric analyses on TA6V and PEEK implants and microcomputed tomography on PEEK implants revealed that the bone-to-implant contact and bone area around the implants were significantly lower for the BMP-2-coated implants than for the bare implants. This was confirmed by scanning electron microscopy imaging. This difference was more pronounced at 4weeks in comparison to the 8-week time point. However, bone growth inside the hexagonal upper hollow cavity of the screws was higher in the case of the BMP-2 coated implants. Overall, this study shows that a high dose of BMP-2 leads to localized and temporary bone impairment, and that the dose of BMP-2 delivered at the surface of an implant needs to be carefully optimized.
The presentation of growth factors from material surfaces currently presents significant challenges in academia, clinics and industry. Applying osteoinductive factors to different types of implants, made of metals or polymers, may improve bone repair in difficult situations. Here, we show the effects of an osteoinductive coating made of polyelectrolyte multilayer films on two widely used materials, titanium TA6V alloys and PEEK implants, which were implanted in the rabbit femoral condyle. We show that a too high dose of BMP-2 delivered from the screw surface has a negative short-term effect on bone regeneration in close vicinity of the screw surface. In contrast, bone formation was increased at early times in the empty spaces around the screw. These results highlight the need for future dose-dependence studies on bone formation in response to osteoinductive coatings.
本研究的目的是评估由聚电解质多层膜制成的递送骨形态发生蛋白-2（BMP-2）的表面涂层诱导的钛植入物（Ti-6Al-4V，此处记为TA6V）和聚醚醚酮（PEEK）植入物的骨整合情况。使用碱性磷酸酶测定法评估聚电解质膜涂层植入物的体外生物活性。将总剂量为9.3μg BMP-2的BMP-2涂层TA6V和PEEK植入物插入新西兰白兔的股骨髁，并与未涂层的植入物进行比较。植入后4周和8周处死兔子。对TA6V和PEEK植入物进行组织形态计量学分析，对PEEK植入物进行微计算机断层扫描，结果显示，BMP-2涂层植入物的骨与植入物接触面积和植入物周围的骨面积显著低于未涂层植入物。扫描电子显微镜成像证实了这一点。与8周时间点相比，这种差异在4周时更为明显。然而，在BMP-2涂层植入物的情况下，螺钉六边形上部中空腔内的骨生长更高。总体而言，本研究表明高剂量的BMP-2会导致局部和暂时性的骨损伤，并且需要仔细优化植入物表面递送的BMP-2剂量。
从材料表面呈现生长因子目前在学术界、临床和工业界都面临重大挑战。将骨诱导因子应用于由金属或聚合物制成的不同类型植入物，可能会改善困难情况下的骨修复。在此，我们展示了由聚电解质多层膜制成的骨诱导涂层对两种广泛使用的材料——钛TA6V合金和PEEK植入物（植入兔股骨髁）的影响。我们表明，从螺钉表面递送的过高剂量的BMP-2对螺钉表面附近的骨再生有负面短期影响。相比之下，在螺钉周围的空隙中，早期骨形成增加。这些结果凸显了未来针对骨诱导涂层对骨形成的剂量依赖性研究的必要性。
