Department of Polymer Engineering, University of Akron, Ohio 44325-0301, United States.
Biomacromolecules. 2013 May 13;14(5):1423-33. doi: 10.1021/bm4000794. Epub 2013 Apr 17.
Genistein is a phytochemical with a broad range of desirable biological activity for wound healing. However, its poor bioavailability requires developing a new method for fabricating an appropriate carrier vehicle to deliver genistein in a sustained manner. Based on the guidance afforded by the ternary phase diagram of poly(D,L-lactic acid) (PDLLA), poly(ethylene oxide) (PEO), and genistein blends, certain selective compositions were electrospun. We obtained a uniformly smooth surface morphology in unmodified and genistein-modified PEO/PDLLA fibers, documented by scanning electron microscopy. Moreover, wide-angle X-ray diffraction and 1H NMR studies revealed that the genistein molecules, successfully incorporated in the blends, remained chemically stable after electrospinning. Besides surface wettability and dimensional stability of the electrospun mats, the released genistein amount has been evaluated as a function of PEO concentration. Our biocompatibility investigations suggest that genistein-modified PEO/PDLLA electrospun mats exhibit strong antioxidant and anti-inflammatory activities which indicate they have potential applications for wound dressings.
染料木黄酮是一种具有广泛理想生物活性的植物化学物质，可用于伤口愈合。然而，其生物利用度差，需要开发新的方法来制备合适的载体，以持续输送染料木黄酮。基于聚（D，L-乳酸）（PDLLA）、聚（氧化乙烯）（PEO）和染料木黄酮共混物三元相图的指导，对某些选择性成分进行了静电纺丝。我们通过扫描电子显微镜获得了未经修饰和染料木黄酮修饰的 PEO/PDLLA 纤维的均匀光滑表面形态。此外，广角 X 射线衍射和 1H NMR 研究表明，成功掺入共混物中的染料木黄酮分子在静电纺丝后仍保持化学稳定。除了静电纺丝垫的表面润湿性和尺寸稳定性外，还评估了 PEO 浓度对释放的染料木黄酮量的影响。我们的生物相容性研究表明，染料木黄酮修饰的 PEO/PDLLA 静电纺丝垫具有很强的抗氧化和抗炎活性，表明它们在伤口敷料方面有潜在的应用。
