Green synthesis of a lactoferrin-infused silver nanoparticle gel for enhanced wound healing.

The study analyzed the benefits of nano-silver (AgNPs) in reducing side effects and enhancing efficacy, highlighting the advantages compared to silver ions. The study examined the production of AgNPs-lactoferrin complexes (AgNPs-LTF) using bovine lactoferrin (LTF) at 1, 2, and 4 mM concentrations. The objective was to create an AgNPs-LTF gel with Carbopol as the base and assess its effectiveness in enhancing wound healing in rats. UV-Vis, PL, FTIR, and XRD analyses confirmed the synthesis of AgNPs. Microscopic examinations (TEM and SEM) showed mainly spherical AgNPs in the AgNPs-LTF samples, with diameters between 11 and 27 nm. The AgNPs-LTF gel with biologically processed AgNPs demonstrated effective infection control and enhanced wound healing outcomes. In Sprague-Dawley rats, the 4 mM AgNPs-LTF gel demonstrated significant wound closure, achieving complete closure by day 10, exceeding the healing rates of both the LTF and control groups. The AgNPs-LTF complex demonstrated high robustness and exceeded the performance of native LTF, exhibiting similar toxicity levels to AgNPs. The study shows the effectiveness of AgNPs-LTF gel in wound treatment, indicating its potential as a viable treatment option.