Lowe A, Bills J, Verma R, Lavery L, Davis K, Balkus K J
Department of Chemistry, University of Texas at Dallas, 800 West Campbell Road, Mailstop BE26, Richardson, TX 75080-3021, USA.
Department of Plastic Surgery, University of Texas at Southwestern, 5323 Harry Hines Blvd, Dallas, TX 75390, USA.
Acta Biomater. 2015 Feb;13:121-30. doi: 10.1016/j.actbio.2014.11.032. Epub 2014 Nov 23.
Research has shown that nitric oxide (NO) enhances wound healing. The incorporation of NO into polymers for medical materials and surgical devices has potential benefits for many wound healing applications. In this work, acrylonitrile (AN)-based terpolymers were electrospun to form non-woven sheets of bandage or wound dressing type materials. NO is bound to the polymer backbone via the formation of a diazeniumdiolate group. In a 14 day NO release study, the dressings released 79 μmol NO g(-1) polymer. The NO-loaded dressings were tested for NO release in vivo, which demonstrate upregulation of NO-inducible genes with dressing application compared to empty dressings. Studies were also conducted to evaluate healing progression in wounds with dressing application performed weekly and daily. In two separate studies, excisional wounds were created on the dorsa of 10 mice. Dressings with NO loaded on the fibers or empty controls were applied to the wounds and measurements of the wound area were taken at each dressing change. The data show significantly enhanced healing progression in the wounds with weekly NO application, which is more dramatic with daily application. Further, the application of daily NO bandages results in improved wound vascularity. These data demonstrate the potential for this novel NO-releasing dressing as a valid wound healing therapy.
研究表明，一氧化氮（NO）可促进伤口愈合。将NO掺入用于医疗材料和手术器械的聚合物中，对许多伤口愈合应用具有潜在益处。在这项工作中，基于丙烯腈（AN）的三元共聚物通过静电纺丝形成绷带或伤口敷料类型材料的无纺布。NO通过形成二醇二氮烯基团与聚合物主链结合。在一项为期14天的NO释放研究中，敷料释放出79 μmol NO g(-1)聚合物。对负载NO的敷料进行了体内NO释放测试，结果表明与空白敷料相比，使用该敷料可使NO诱导基因上调。还进行了研究以评估每周和每天使用敷料时伤口的愈合进程。在两项独立研究中，在10只小鼠的背部制造切除伤口。将纤维上负载有NO的敷料或空白对照应用于伤口，并在每次更换敷料时测量伤口面积。数据显示，每周使用NO可显著增强伤口的愈合进程，每天使用则效果更显著。此外，每天使用NO绷带可改善伤口血管生成。这些数据证明了这种新型NO释放敷料作为一种有效的伤口愈合疗法的潜力。
