Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Macromol Biosci. 2013 Jun;13(6):789-98. doi: 10.1002/mabi.201300037. Epub 2013 Apr 17.
Fluorescein isothiocyanate (FITC), a fluorescent probe, is coupled to amphiphilic monomethoxy poly(ethylene glycol)-block-poly(ε-caprolactone) (mPEG-PCL) copolymers. FITC-labeled mPEG-PCL copolymers self-assemble into micelles through the solvent evaporation method. The cellular internalization is examined using fluorescence microscopy on incubation of NIH-3T3 fibroblasts with micelles or free FITC solution. The effect of the hydrophilic/hydrophobic ratio on the endocytosis mechanisms is evaluated by fluorescence microscopy on culturing of human hepatoblastoma cells and human umbilical vein endothelial cells, individually, mixed with the micelles holding the same parameters including micelle size, shape, and surface charges.
荧光素异硫氰酸酯（FITC）是一种荧光探针，与两亲性单甲氧基聚（乙二醇）-嵌段-聚（ε-己内酯）（mPEG-PCL）共聚物偶联。FITC 标记的 mPEG-PCL 共聚物通过溶剂蒸发法自组装成胶束。通过将 NIH-3T3 成纤维细胞与胶束或游离 FITC 溶液孵育，用荧光显微镜检查细胞内化。通过单独培养人肝癌细胞和人脐静脉内皮细胞，并将其与具有相同参数（包括胶束大小、形状和表面电荷）的胶束混合，用荧光显微镜评估亲水/亲脂比对细胞内吞机制的影响。
