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文旦籽酸性多糖的理化性质及抗氧化活性。

Physicochemical properties and antioxidant activities of acidic polysaccharides from wampee seeds.

机构信息

College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.

出版信息

Int J Biol Macromol. 2013 Aug;59:90-5. doi: 10.1016/j.ijbiomac.2013.04.020. Epub 2013 Apr 15.

Abstract

Two novel polysaccharide fractions, named WP1 and WP3, were extracted and purified from wampee [Clausena lansium (Lour.) Skeels] seeds. Their physicochemical properties, in vitro and in vivo antioxidant activities were investigated. Results indicated that both WP1 and WP3 were acidic heteropolysaccharides with uronic acid content of 45.35% and 56.79%, respectively. The molar ratio of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose of WP1 was 5.01:1.50:39.53:1:10.76:23.50 while WP3 was 5.76:1.73:27.27:1:4.72:8.77. Furthermore, WP3 not only exhibited stronger in vitro antioxidant activities than WP1, but also more significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) level in serum of SD rats induced by high-fat diet.

摘要

从沃柑[Clausena lansium (Lour.) Skeels]种子中提取并纯化出两种新型多糖组分 WP1 和 WP3。研究了它们的理化性质、体外和体内抗氧化活性。结果表明,WP1 和 WP3 均为酸性杂多糖,其糖醛酸含量分别为 45.35%和 56.79%。WP1 的鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖摩尔比为 5.01:1.50:39.53:1:10.76:23.50,WP3 则为 5.76:1.73:27.27:1:4.72:8.77。此外,WP3 不仅表现出比 WP1 更强的体外抗氧化活性,而且还显著提高了高脂肪饮食诱导的 SD 大鼠血清中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平的活性。

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