State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta. 2013 Mar 30;107:55-60. doi: 10.1016/j.talanta.2012.12.055. Epub 2013 Jan 5.
A novel, sensitive and enzymeless electrochemical sensor based on polynucleotide-templated silver nanoclusters (DNA-AgNCs)/graphene composite film was developed for the detection of hydrogen peroxide. The graphene modified glassy carbon electrode (GCE) was employed because graphene has several advantages including excellent conductivity, biocompatibility, and large surface area to volume ratio. In addition, it was found that DNA-AgNCs have remarkable electrocatalytic activity toward the reduction of hydrogen peroxide, and can be easily immobilized onto the surface of the graphene/GCE by π-π stacking. The sensor based on the (DNA-AgNCs)/graphene/GCE exhibited a rapid response (ca. 3s), a low detection limit (3 μM), a wide linear range from 15 μM to 23 mM, high selectivity, as well as good repeatability. Moreover, the common interfering species, such as ascorbic acid, uric acid, dopamine, glutathione, and l-cysteine, did not result in any interference. This present work may expand the use of silver nanoclusters in the field of electrochemical sensor.
一种基于多核苷酸模板银纳米簇（DNA-AgNCs）/石墨烯复合膜的新型、灵敏、无酶电化学传感器被开发用于检测过氧化氢。由于石墨烯具有优良的导电性、生物相容性和大的表面积与体积比等优点，因此选用了修饰有石墨烯的玻碳电极（GCE）。此外，研究发现 DNA-AgNCs 对过氧化氢的还原具有显著的电催化活性，并且可以通过π-π 堆积容易地固定在石墨烯/GCE 的表面上。基于（DNA-AgNCs）/石墨烯/GCE 的传感器表现出快速的响应（约 3s）、低的检测限（3μM）、从 15μM 到 23mM 的宽线性范围、高的选择性以及良好的重现性。此外，常见的干扰物质，如抗坏血酸、尿酸、多巴胺、谷胱甘肽和半胱氨酸，不会导致任何干扰。本工作可能会扩展银纳米簇在电化学传感器领域的应用。
