Goodlad G A, Ma G Y
Biochim Biophys Acta. 1975 Jan 20;378(2):221-9. doi: 10.1016/0005-2787(75)90110-0.
Studies were undertaken on the turnover of ribosomal RNA and on ribonuclease activity in the liver of the pregnant rat in an attempt to explain the accumulation of liver RNA which occurs during the latter half of pregnancy. Between the 15th and 20th day of gestation the rate constant of degradation, biological half-life and daily rate of synthesis of ribosomal RNA were calculated to be 0.0887, 7.81 days and 6.21 mg per liver per 100g body weight respectively. Corresponding values in non-pregnant rats were 0.123, 5.68 days and 3.47 mg per liver per 100g body weight. The increase in RNA was therefore associated with an increase in its rate of synthesis and a decrease in its rate of breakdown. From the 14th day of pregnancy there was a decrease in alkaline ribonuclease activity and a marked increase in the level of alkaline ribonuclease inhibitor. The activity of acid ribonuclease was found to increase and that of acid phosphatase to decrease during this period.
为了解释妊娠后期大鼠肝脏中RNA积累的现象，对妊娠大鼠肝脏中核糖体RNA的周转和核糖核酸酶活性进行了研究。在妊娠第15至20天期间，核糖体RNA的降解速率常数、生物学半衰期和每日合成速率经计算分别为0.0887、7.81天和每100克体重肝脏6.21毫克。非妊娠大鼠的相应数值分别为0.123、5.68天和每100克体重肝脏3.47毫克。因此，RNA的增加与其合成速率的增加和分解速率的降低有关。从妊娠第14天起，碱性核糖核酸酶活性降低，碱性核糖核酸酶抑制剂水平显著升高。在此期间，酸性核糖核酸酶的活性增加，而酸性磷酸酶的活性降低。
