Xie Lei-Rui, Li Dan-Yi, Wang Pei-Le, Hua Hui-Ming, Wu Xin, Li Zhan-Lin
Key Laboratory of Structure-Based Drugs Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China.
Yao Xue Xue Bao. 2013 Jan;48(1):89-93.
To study the secondary metabolites of a marine-derived fungus Ascotricha sp. ZJ-M-5, several chromatographic methods including macroporous resin, silica gel, ODS and Sephadex LH-20 were used to isolate the compounds, and their structures were elucidated on the basis of physicochemical properties and spectroscopic methods. Ten compounds were obtained and identified as ascotrichic acid B (1), (3R)-6-hydroxymellein (2), beta-carboline (3), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol (4), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta, 9alpha-tetraol (5), cyclo (Leu-Pro) (6), cyclo (Ile-Leu) (7), cyclo (Pro-Val) (8), cyclo (Pro-Gly) (9), and cyclo (Hpro-Ala) (10). Among them, compound 1 is a new 3, 4-seco-lanostane triterpenoid which has been isolated from the filamentous fungi for the first time, and compounds 2-10 are firstly isolated from Ascotricha genus.
为研究海洋来源真菌Ascotricha sp. ZJ-M-5的次生代谢产物，采用大孔树脂、硅胶、ODS和葡聚糖凝胶LH-20等多种色谱方法分离化合物，并根据其理化性质和光谱方法阐明其结构。共获得10个化合物，分别鉴定为曲霉酸B（1）、（3R）-6-羟基水合卟啉（2）、β-咔啉（3）、（22E，24R）-麦角甾-7，22-二烯-3β，5α，6β-三醇（4）、（22E，24R）-麦角甾-7，22-二烯-3β，5α，6β，9α-四醇（5）、环（亮氨酸-脯氨酸）（6）、环（异亮氨酸-亮氨酸）（7）、环（脯氨酸-缬氨酸）（8）、环（脯氨酸-甘氨酸）（9）和环（羟脯氨酸-丙氨酸）（10）。其中，化合物1是一种新的3，4-裂环羊毛甾烷三萜类化合物，首次从丝状真菌中分离得到，化合物2-10首次从曲霉属中分离得到。
