[青稞β-葡聚糖对高脂诱导C57小鼠血糖及血脂的影响]

[Effects of beta glucan in highland barley on blood glucose and serum lipid in high fat-induced C57 mouse].

作者信息

Tian Ming-jie, Song Jiang-nan, Liu Pei-pei, Su Li-hong, Sun Chang-hao, Li Ying

机构信息

Department of Nutrition and Food Hygiene, Harbin Medical University, Harbin 150081, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2013 Jan;47(1):55-8.

PMID:23601524

Abstract

OBJECTIVE

Study the effects of β-glucan in highland barley on blood glucose and serum lipid in high fat diet induced C57 mouse.

METHODS

Using table of random number, 40 male C57BL/6 mice were randomly divided into 4 groups (10 mice in each group) by weight: high dosage group (4% β-glucan and high fat diet), low dosage group (2% β-glucan and high fat diet), high fat diet group and normal control group. Food-intake and body weight of C57 mouse were observed. Glucose tolerance tests and examinations of fasting blood glucose were performed at the end of 11 weeks of intervention. Mice were sacrificed after 12 wk of treatment, and serum specimens were obtained to test relevant biochemical indicators.

RESULTS

After 12 weeks raise, among high dosage group, low dosage group, high fat diet group and normal control group, the weight was (32.8 ± 1.5), (40.4 ± 1.9), (40.7 ± 2.1) and (33.5 ± 1.3) g, respectively (F = 55.26, P < 0.05); average food intake was (3.48 ± 0.56), (3.69 ± 0.76), (3.66 ± 0.81) and (3.54 ± 0.61) g/d respectively (F = 0.26, P > 0.05); fasting blood-glucose was (5.29 ± 1.59), (6.13 ± 1.75), (7.63 ± 1.09) and (4.24 ± 0.98) mmol/L respectively (F = 9.54, P < 0.01); serum insulin level was (1.97 ± 0.10), (2.44 ± 0.24), (3.02 ± 0.36) and (1.48 ± 0.28) ng/ml respectively (F = 47.58, P < 0.01); the area under blood glucose concentration curve was (25.81 ± 1.44), (30.42 ± 2.01), (35.17 ± 1.20) and (21.03 ± 1.24) mmol×L(-1)×h(-1), respectively (F = 64.98, P < 0.05); insulin resistance index was (9.84 ± 3.78), (13.69 ± 4.48), (21.54 ± 3.27) and (5.81 ± 1.59) respectively (F = 30.18, P < 0.01); serum total cholesterol (TC) level was (4.05 ± 0.88), (4.30 ± 0.48), (4.73 ± 0.66) and (3.37 ± 0.40) mmol/L respectively (F = 6.70, P < 0.01); serum triglyceride (TG) level was (0.90 ± 0.09), (0.98 ± 0.09), (1.05 ± 0.06) and (0.76 ± 0.26) mmol/L respectively (F = 6.75, P < 0.01); serum high-density lipoprotein cholesterol (HDL-C) level was (2.91 ± 0.59), (3.34 ± 0.46), (4.89 ± 0.42) and (3.24 ± 0.37) mmol/L respectively (F = 31.73, P < 0.01); serum low-density lipoprotein cholesterol (LDL-C) level was (0.25 ± 0.15), (0.42 ± 0.19), (0.72 ± 0.12) and (0.32 ± 0.11) mmol/L, respectively (F = 17.27, P < 0.01); free fatty acids (FFA) level was (1.06 ± 0.03), (1.05 ± 0.05), (1.18 ± 0.32) and (1.04 ± 0.02) mmol/L, respectively (F = 1.36, P > 0.05); HDL-C/LDL-C was (13.77 ± 5.51), (9.11 ± 3.53), (7.04 ± 1.65) and (11.21 ± 3.31), respectively (F = 5.24, P < 0.01).

CONCLUSION

The β-glucan in highland barley reduced the serum glucose and serum lipid, as well as insulin resistance and the risk of arterial sclerosis in high-fat induced C57 mouse.

摘要

目的

研究青稞β-葡聚糖对高脂饮食诱导的C57小鼠血糖和血脂的影响。

方法

采用随机数字表法，将40只雄性C57BL/6小鼠按体重随机分为4组（每组10只）：高剂量组（4%β-葡聚糖+高脂饮食）、低剂量组（2%β-葡聚糖+高脂饮食）、高脂饮食组和正常对照组。观察C57小鼠的进食量和体重。干预11周结束时进行葡萄糖耐量试验和空腹血糖检测。治疗12周后处死小鼠，取血清标本检测相关生化指标。

结果

饲养12周后，高剂量组、低剂量组、高脂饮食组和正常对照组小鼠体重分别为（32.8±1.5）、（40.4±1.9）、（40.7±2.1）和（33.5±1.3）g（F=55.26，P<0.05）；平均进食量分别为（3.48±0.56）、（3.69±0.76）、（3.66±0.81）和（3.54±0.61）g/d（F=0.26，P>0.05）；空腹血糖分别为（5.29±1.59）、（6.13±1.75）、（7.63±1.09）和（4.24±0.98）mmol/L（F=9.54，P<0.01）；血清胰岛素水平分别为（1.97±0.10）、（2.44±0.24）、（3.02±0.36）和（1.48±0.28）ng/ml（F=47.58，P<0.01）；血糖浓度曲线下面积分别为（25.81±1.44）、（30.42±2.01）、（35.17±1.20）和（21.03±1.24）mmol×L⁻¹×h⁻¹（F=64.98，P<0.05）；胰岛素抵抗指数分别为（9.84±3.78）、（13.69±4.48）、（21.54±3.27）和（5.81±1.59）（F=30.18，P<0.01）；血清总胆固醇（TC）水平分别为（4.05±0.88）、（4.30±0.48）、（4.73±0.66）和（3.37±0.40）mmol/L（F=6.70，P<0.01）；血清甘油三酯（TG）水平分别为（0.90±0.09）、（0.98±0.09）、（1.05±0.06）和（0.76±0.26）mmol/L（F=6.75，P<0.01）；血清高密度脂蛋白胆固醇（HDL-C）水平分别为（2.91±0.59）、（3.34±0.46）、（4.89±0.42）和（3.24±0.37）mmol/L（F=31.73，P<0.01）；血清低密度脂蛋白胆固醇（LDL-C）水平分别为（0.25±0.15）、（0.42±0.19）、（0.72±0.12）和（0.32±0.11）mmol/L（F=17.27，P<0.01）；游离脂肪酸（FFA）水平分别为（1.06±0.03）、（1.05±0.05）、（1.18±0.32）和（1.04±0.02）mmol/L（F=1.36，P>0.05）；HDL-C/LDL-C分别为（13.77±5.51）、（9.11±3.53）、（7.04±1.65）和（11.21±3.31）（F=5.24，P<0.01）。