State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China.
J Chromatogr A. 2013 May 24;1291:122-8. doi: 10.1016/j.chroma.2013.03.022. Epub 2013 Mar 26.
In this paper, a micro gas chromatography (μGC) column with embedded micro-posts was developed for increasing overall surface area of the columns which is able to support more of the stationary phase and reducing the effective width of the column, leading to higher separation efficiency. The proposed columns have a higher sample capacity as the overall surface area is about 3 times larger than that of open columns with the same dimensions. In order to achieve an even flow velocity in the channels, the location of the micro-posts in the linear channels and the configuration of curved channels were optimized by numerical simulation. The results have indicated that the proposed column separated 5 environmental carcinogens in less than 50s, achieved a separation efficiency of about 9500plates/m and eluted highly symmetrical Gaussian peaks.
本文开发了一种带有嵌入式微柱的微型气相色谱（μGC）柱，用于增加柱的总表面积，从而能够支持更多的固定相，并减小柱的有效宽度，从而提高分离效率。与具有相同尺寸的开放式柱相比，所提出的柱具有更高的样品容量，因为总表面积大约是其 3 倍。为了实现通道中均匀的流速，通过数值模拟优化了线性通道中微柱的位置和弯曲通道的结构。结果表明，所提出的柱在不到 50 秒的时间内分离了 5 种环境致癌物质，实现了约 9500 块/米的分离效率，并洗脱了高度对称的高斯峰。
