Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93053 Regensburg, Germany.
Anal Chim Acta. 2013 May 2;775:1-13. doi: 10.1016/j.aca.2012.12.042. Epub 2013 Jan 4.
This review discusses advances in the field of high resolution scanning electrochemical microscopy (HR-SECM) and scanning ion conductance microscopy (SICM) to study living cells. Relevant references from the advent of this technique in the late 1980s to most recent contributions in 2012 are presented with special discussion on high resolution images. A clear progress especially within the last 5 years can be seen in the field of HR-SECM. Furthermore, we also concentrate on the intrinsic properties of SECM imaging techniques e.g. different modes of image acquisition, their advantages and disadvantages in imaging living cells and strategies for further enhancement of image resolution, etc. Some of the recent advances of SECM in nanoimaging have also been discussed which may have potential applications in high resolution imaging of cellular processes.
本文回顾了近年来在研究活细胞的高分辨率扫描电化学显微镜（HR-SECM）和扫描离子电导显微镜（SICM）领域的进展。本文介绍了自 20 世纪 80 年代末该技术问世以来的相关参考文献，并特别讨论了高分辨率图像。尤其是在过去 5 年内，HR-SECM 领域取得了明显的进展。此外，本文还集中讨论了 SECM 成像技术的固有特性，例如不同的图像采集模式、在活细胞成像中的优缺点以及提高图像分辨率的策略等。本文还讨论了 SECM 在纳米成像方面的一些最新进展，这些进展可能在细胞过程的高分辨率成像中有潜在的应用。
