Department of Virology, Statens Serum Institut, Copenhagen, Denmark.
J Clin Virol. 2013 Jul;57(3):239-42. doi: 10.1016/j.jcv.2013.03.015. Epub 2013 Apr 19.
During the last few years many new human picornaviruses have been discovered due to advances in metagenomics and other molecular biological approaches. The clinical significance and the occurrence are only sparsely described.
To determine the epidemiology and clinical significance of infections with the novel human picornaviruses, aichi virus, cosavirus, salivirus, and saffold virus in infants in Denmark.
We tested 1393 stool samples from a birth cohort of 454 children for these viruses. Samples were collected at ages 6, 10 and 15 months, and at episodes of gastroenteritis. Samples were tested by real-time reverse-transcriptase polymerase chain reaction assays. Each study participant had a diary, where the parents reported episodes of disease, including gastroenteritis.
Aichi virus, salivirus and saffold virus were detected in 6, 9 and 38 of the children, respectively, but cosavirus was not detected in any of the children. There was a clear seasonal variation with most infections occurring in autumn and winter. A statistically significant association between the findings of salivirus and gastrointestinal disease was demonstrated. There was no association between gastrointestinal disease and the presence of aichi virus or saffold virus.
The newly discovered human picornaviruses aichi virus, saffold virus, and salivirus are circulating in Danish children, with the most common being saffold virus. Saffold virus was seen almost exclusively in the autumn and winter period. Salivirus was the only virus, which was significantly associated with gastroenteritis, although the number of positive samples was rather low.
由于宏基因组学和其他分子生物学方法的进步，在过去的几年中发现了许多新的人类小核糖核酸病毒。其临床意义和发生情况仅得到了稀疏的描述。
确定新型人类小核糖核酸病毒，即乙型肝炎病毒、冠状病毒、唾液病毒和沙福病毒在丹麦婴儿中的流行病学和临床意义。
我们使用实时逆转录聚合酶链反应检测了来自 454 名儿童的 1393 份粪便样本，以检测这些病毒。样本采集于 6、10 和 15 个月龄，以及肠胃炎发作时。每位研究参与者都有一份日记，记录父母报告的疾病发作，包括肠胃炎。
乙型肝炎病毒、唾液病毒和沙福病毒分别在 6、9 和 38 名儿童中检测到，但在任何儿童中均未检测到冠状病毒。存在明显的季节性变化，大多数感染发生在秋季和冬季。表明唾液病毒的检测结果与胃肠道疾病存在统计学显著关联。未发现胃肠道疾病与乙型肝炎病毒或沙福病毒的存在存在关联。
新发现的人类小核糖核酸病毒乙型肝炎病毒、沙福病毒和唾液病毒在丹麦儿童中传播，最常见的是沙福病毒。沙福病毒仅在秋季和冬季出现。唾液病毒是唯一与肠胃炎显著相关的病毒，尽管阳性样本数量较低。
