Yu W M, Coddington D, Bitter-Suermann H
Department of Surgery, Dalhousie University, Halifax, Nova Scotia, Canada.
Transplantation. 1990 Jun;49(6):1060-6. doi: 10.1097/00007890-199006000-00007.
A total of 278 orthotopic rat liver grafts, without arterialization, were performed, in an attempt to determine which of the individual components of UW solution are essential. Livers were preserved by in situ flushing and cold storage with the following results: 56% of rats survived for 1 week after 9 hr of preservation with UW solution as compared with 44% using Marshall solution, and 10% using Collins solution. Having established LD 50 for UW solution, we then omitted its components one at a time and found that omission of HES, raffinose, allopurinol, adenosine, phosphate buffer, or MgSO4 did not change survival after 9 hr of preservation. Omission of lactobionate, glutathione, and dexamethasone, respectively, resulted in decreased survival, whereas elimination of insulin surprisingly increased survival. In ensuing dose-response studies, the concentrations of lactobionate, glutahione, dexamethasone in UW solution proved to be optimal. Finally, livers were preserved with a solution containing only lactobionate, glutathione, dexamethasone, raffinose, and phosphate buffer, resulting in 53% animal survival, as compared with 56% for the unchanged UW solution. We conclude that UW solution can be simplified without loss of effectiveness in this model.
共进行了278例未进行动脉化的原位大鼠肝移植，以确定UW液的各个成分中哪些是必不可少的。通过原位冲洗和冷藏保存肝脏，结果如下：用UW液保存9小时后，56%的大鼠存活1周，而使用马歇尔液的为44%，使用柯林斯液的为10%。确定了UW液的半数致死剂量（LD50）后，我们接着逐一去除其成分，发现去除羟乙基淀粉（HES）、棉子糖、别嘌呤醇、腺苷、磷酸盐缓冲液或硫酸镁后，保存9小时后的存活率没有变化。分别去除乳糖酸盐、谷胱甘肽和地塞米松会导致存活率降低，而去除胰岛素出人意料地提高了存活率。在随后的剂量反应研究中，UW液中乳糖酸盐、谷胱甘肽、地塞米松的浓度被证明是最佳的。最后，用仅含乳糖酸盐、谷胱甘肽、地塞米松、棉子糖和磷酸盐缓冲液的溶液保存肝脏，动物存活率为53%，而未改变的UW液为56%。我们得出结论，在该模型中UW液可以简化而不丧失有效性。
