Kuratsu J, Sueyoshi N, Mihara Y, Ushio Y
Department of Neurosurgery, Kumamoto University Medical School, Japan.
Acta Neuropathol. 1990;79(6):634-9. doi: 10.1007/BF00294241.
It has been suggested that peanut agglutinin (PNA)-binding sites in benign and malignant tissues were quite different. To clarify the difference of PNA-binding sites between benign and malignant ependymoma, PNA-binding sites are investigated on the surface membrane of tissues from benign and malignant ependymomas and cultured ependymoma cells. In four of five malignant ependymoma cases, PNA binding occurred in a diffuse cell membrane fashion or granular intracytoplasmic fashion without neuraminidase treatment. On the other hand, PNA binding was observed without neuraminidase treatment in only two of eight benign ependymoma cases. After neuraminidase treatment, all of our benign and malignant ependymoma cases evidenced PNA binding. PNA binding was clearly evident on the cell membrane of ependymoma cells which were able to express organotypic structures, that is, to form ependymal rosettes after neuraminidase treatment. Normal rat ependymal cells showed PNA binding only after neuraminidase treatment. These findings suggest that the masking of PNA-binding sites of ependymoma cells by sialic acid may be correlated with tumor cell differentiation.
有人提出，良性和恶性组织中花生凝集素（PNA）结合位点存在很大差异。为了阐明良性和恶性室管膜瘤中PNA结合位点的差异，对良性和恶性室管膜瘤组织以及培养的室管膜瘤细胞的表面膜上的PNA结合位点进行了研究。在5例恶性室管膜瘤病例中的4例中，未经神经氨酸酶处理时，PNA以弥漫性细胞膜方式或颗粒状胞浆内方式结合。另一方面，在8例良性室管膜瘤病例中，只有2例在未经神经氨酸酶处理时观察到PNA结合。经神经氨酸酶处理后，我们所有的良性和恶性室管膜瘤病例均显示PNA结合。在能够表达器官样结构的室管膜瘤细胞的细胞膜上，即经神经氨酸酶处理后形成室管膜玫瑰花结的细胞膜上，PNA结合明显可见。正常大鼠室管膜细胞仅在经神经氨酸酶处理后显示PNA结合。这些发现表明，唾液酸对室管膜瘤细胞PNA结合位点的掩盖可能与肿瘤细胞分化相关。
