Schwechheimer K, Schnabel P, Möller P
Acta Neuropathol. 1983;61(1):21-6. doi: 10.1007/BF00688382.
Peroxidase-antiperoxidase (PAP) technique was selected for visualizing the binding of peanut lectin (PNL) to the most frequent human brain tumors. The randomly selected material included neoplasms of neuroectodermal and mesenchymal origin. We employed 1--5 micrometers of routinely processed and paraffin-embedded tissues. PNL receptors were detected to a variable extent on the cell surface of astrocytoma, glioblastoma multiforme, oligodendroglioma, ependymoma, meningotheliomatous meningeoma, and plexus papilloma. In gliomas as increase in malignancy seems to be associated with a decrease in PNL binding. Except for the plexus papillomas, neuraminidase pretreatment had neither a qualitative nor a quantitative influence on the binding behavior of PNL. Intracellular PNL receptors could be detected in "granular cells" and in the perinuclear region of malignant gliomas.
采用过氧化物酶-抗过氧化物酶（PAP）技术来观察花生凝集素（PNL）与最常见的人类脑肿瘤的结合情况。随机选取的材料包括神经外胚层和间充质来源的肿瘤。我们使用了常规处理并石蜡包埋的1至5微米厚的组织。在星形细胞瘤、多形性胶质母细胞瘤、少突胶质细胞瘤、室管膜瘤、脑膜皮瘤型脑膜瘤和丛状乳头状瘤的细胞表面不同程度地检测到了PNL受体。在胶质瘤中，恶性程度的增加似乎与PNL结合的减少有关。除了丛状乳头状瘤外，神经氨酸酶预处理对PNL的结合行为在定性和定量方面均无影响。在“颗粒细胞”和恶性胶质瘤的核周区域可检测到细胞内PNL受体。
