HPLC 法分离土曲霉次级代谢产物。

Isolation of secondary metabolites of Aspergillus ochraceus by HPLC.

机构信息

Departamento de Quimica, Universidade de Aveiro, 3800, Aveiro, Portugal.

出版信息

Mycotoxin Res. 1986 Mar;2(1):9-17. doi: 10.1007/BF03191957.

DOI:10.1007/BF03191957

Abstract

A method is described for the isolation and purification of ochratoxin A, ochratoxin B, ochratoxin ß mellein, 4-hydroxymellein and penicillic acid produced byAspergillus ochraceus in a synthetic liquid medium. Ochratoxin α, which was not found in the culture medium, was obtained by acid hydrolysis of ochratoxin A. A high pressure liquid Chromatograph equipped with Lichrosorb 100 and Lichrosorb RP-18 columns and UV and/or Refractive Index detectors was used.

摘要

描述了用一种方法从合成液体培养基中分离和提纯由赭曲霉产生的赭曲霉毒素 A、B、β 麦角甾醇、4-羟麦角甾醇和青霉素。由于在培养基中未发现赭曲霉毒素 α，所以用酸水解赭曲霉毒素 A 得到它。使用高压液相色谱，配有 Lichrosorb 100 和 Lichrosorb RP-18 柱以及紫外和/或折射指数检测器。