Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, 466-8555, Japan.
J Mater Sci Mater Med. 2013 Jul;24(7):1649-58. doi: 10.1007/s10856-013-4930-5. Epub 2013 Apr 20.
Cotton wool-like poly(L-lactic acid) and siloxane-doped vaterite (SiV) composite scaffolds were prepared with a modified electrospinning system for bone tissue engineering applications. The effects of changing the SiV content in the materials from 10 to 30 wt% on elasticity and the ability to release calcium ions and soluble silica were evaluated. The elasticity of the cotton wool-like composites was almost the same as that of the PLLA from the results of compressibility and recovery tests. The materials released calcium ions for more than 56 days and soluble silica for 28-56 days in a tris buffer solution (pH 7.4). Mouse osteoblast-like cells (MC3T3-E1 cells) were cultured on/in the cotton wool-like materials or the fibremats out of the same composite materials as that used for the cotton wool-like materials. The cells penetrated into and proliferated inside the cotton wool-like materials, although they mainly adhered on the fibremat surface.
采用改良静电纺丝系统制备了类似棉花的聚（L-乳酸）和硅氧烷掺杂文石（SiV）复合支架，用于骨组织工程应用。评估了材料中 SiV 含量从 10 到 30wt%变化对弹性和释放钙离子和可溶性硅能力的影响。从压缩性和恢复性测试的结果来看，类似棉花的复合材料的弹性几乎与 PLLA 相同。在 tris 缓冲溶液（pH 7.4）中，材料释放钙离子超过 56 天，释放可溶性硅超过 28-56 天。将鼠成骨样细胞（MC3T3-E1 细胞）培养在类似棉花的材料上或由类似棉花的复合材料制成的纤维垫上。细胞穿透并在类似棉花的材料内部增殖，尽管它们主要附着在纤维垫表面。
