Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3617-3622. doi: 10.1099/ijs.0.052738-0. Epub 2013 Apr 19.
A Gram-staining-negative, chemoheterotrophic, yellow-pigmented, non-motile, flexirubin-negative, facultatively anaerobic bacterium, designated strain IMCC3317(T), was isolated from a coastal seawater sample from the Antarctic Penninsula. Optimal growth of strain IMCC3317(T) was observed at 20 °C, pH 8.0 and in the presence of 2-3 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMCC3317(T) belonged to the genus Kordia and was closely related to Kordia algicida OT-1(T) (96.7 % sequence similarity) and Kordia periserrulae IMCC1412(T) (96.1 % sequence similarity). The major fatty acids were 10-methyl C16 : 0 and/or iso-C16 : 1ω9c, iso-C17 : 0 3-OH, iso-C15 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 35.1 mol%. The strain contained menaquinone-6 (MK-6) as the respiratory quinone. The polar lipids detected in the strain were phosphatidylethanolamine and unknown aminophospholipids, aminolipids and polar lipids. On the basis of phylogenetic distinction and differential phenotypic characteristics, it is suggested that strain IMCC3317(T) ( = KCTC 32292(T) = NBRC 109401(T)) be assigned to the genus Kordia as the type strain of a novel species, for which the name Kordia antarctica sp. nov. is proposed.
一株革兰氏阴性、化能异养、黄色、非运动、不含腐臭素、兼性厌氧的细菌，被命名为 IMCC3317(T) 菌株，从南极半岛的沿海海水样本中分离得到。IMCC3317(T) 菌株的最佳生长条件为 20°C、pH8.0 和 2-3%NaCl。基于 16S rRNA 基因序列的系统发育分析表明，IMCC3317(T) 菌株属于 Kordia 属，与 Kordia algicida OT-1(T)（96.7%序列相似性）和 Kordia periserrulae IMCC1412(T)（96.1%序列相似性）最为密切。主要脂肪酸为 10-甲基 C16:0 和/或 iso-C16:1ω9c、iso-C17:0 3-OH、iso-C15:0 和 anteiso-C15:0。基因组 DNA 的 G+C 含量为 35.1mol%。该菌株含有menaquinone-6 (MK-6) 作为呼吸醌。该菌株中检测到的极性脂为磷脂酰乙醇胺和未知的氨基磷脂、氨基脂和极性脂。基于系统发育差异和表型特征的差异，建议将 IMCC3317(T) 菌株（ = KCTC 32292(T) = NBRC 109401(T)）归为 Kordia 属，作为一个新种的模式株，命名为 Kordia antarctica sp. nov.。
