Qi Feng, Huang Zhaobin, Lai Qiliang, Li Dengfeng, Shao Zongze
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.
Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2016 Jul;66(7):2623-2628. doi: 10.1099/ijsem.0.001098. Epub 2016 Apr 20.
A novel bacterial strain, SC2, was isolated from Ulva sp., a green marine algae, which was collected from Dadeng island, Xiamen, China. Strain SC2 was Gram-stain-negative, aerobic, rod-shaped and had no flagellum. Oxidase and catalase were positive. Strain SC2was able to degrade skimmed milk, agar, soluble starch, Tween 20 and Tween 80 but not colloidal chitin. Growth was observed at salinities of 1-6 % and temperatures of 15-37 °C, with optimal salinity and temperature of 2 % and 30 °C, respectively. Phylogenetic analysis based on the 16S rRNA gene indicated that strain SC2 was affiliated to the genus Kordia, with highest sequence similarity to Kordia algicida OT-1 (97.23 %), Kordia antarctica IMCC 3317 (97.23 %) and Kordia jejudonensis SSK3-3 (97.02 %); the type strains of other species of the genus Kordia shared 93.98-95.78 % gene sequence similarity with strain SC2. The average nucleotide identity value and estimated DNA-DNA hybridization value between strain SC2 and the above three type strains (K. algicida OT-1, K. antarctica IMCC 3317 and K. jejudonensis SSK3-3) were found to be 79.4-82.4 % and 24.2-27.0 %, respectively. The predominant fatty acids (>5.0 %) were C16 : 0, iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c), summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c) and summed feature 9 (comprising iso-C17 : 1ω9c/C16 : 0 10-methyl). The respiratory quinone was determined to be solely menaquinone-6 (MK-6). The polar lipid profile of strain SC2 consisted of four unknown lipids, three unidentified phospholipids, one unidentified aminolipid and phosphatidylethanolamine. The G+C content of the genomic DNA was 34.5 mol%. The combined genotypic and phenotypic data showed that strain SC2 represents a novel species within the genus Kordia, for which the name Kordia ulvae sp. nov. is proposed. The type strain is SC2 (= KCTC 42872=MCCC 1A01772=LMG 29123).
从中国厦门大嶝岛采集的绿色海藻石莼中分离出一株新型细菌菌株SC2。菌株SC2革兰氏染色阴性，好氧，杆状，无鞭毛。氧化酶和过氧化氢酶呈阳性。菌株SC2能够降解脱脂牛奶、琼脂、可溶性淀粉、吐温20和吐温80，但不能降解胶体几丁质。在盐度为1%-6%、温度为15-37℃时可观察到生长，最适盐度和温度分别为2%和30℃。基于16S rRNA基因的系统发育分析表明，菌株SC2隶属于科尔迪氏菌属，与杀藻科尔迪氏菌OT-1（97.23%）、南极科尔迪氏菌IMCC 3317（97.23%）和济州岛科尔迪氏菌SSK3-3（97.02%）的序列相似性最高；科尔迪氏菌属其他物种的模式菌株与菌株SC2的基因序列相似性为93.98%-95.78%。发现菌株SC2与上述三种模式菌株（杀藻科尔迪氏菌OT-1、南极科尔迪氏菌IMCC 3317和济州岛科尔迪氏菌SSK3-3）之间的平均核苷酸同一性值和估计的DNA-DNA杂交值分别为79.4%-82.4%和24.2%-27.0%。主要脂肪酸（>5.0%）为C16:0、异C15:0、异C15:0 3-OH、异C17:0 3-OH、特征性总和3（包含C16:1ω7c/C16:1ω6c）、特征性总和8（包含C18:1ω7c/C18:1ω6c）和特征性总和9（包含异C17:1ω9c/C16:0 10-甲基）。测定呼吸醌仅为甲基萘醌-6（MK-6）。菌株SC2的极性脂质谱由四种未知脂质、三种未鉴定的磷脂、一种未鉴定的氨基脂质和磷脂酰乙醇胺组成。基因组DNA的G+C含量为34.5 mol%。综合基因型和表型数据表明，菌株SC2代表科尔迪氏菌属内的一个新物种，为此提出名称石莼科尔迪氏菌（Kordia ulvae sp. nov.）。模式菌株为SC2（=KCTC 42872=MCCC 1A01772=LMG 29123）。
