Department of Cariology, Institute of Odontology, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden; Public Dental Health Service Västra Götaland, University Clinics, Gothenburg, Sweden.
J Oral Pathol Med. 2013 Oct;42(9):691-7. doi: 10.1111/jop.12069. Epub 2013 Apr 23.
Impairment of cellular immunity is reported in lichen planus, an autoimmune disease affecting mucosae and skin. Our aim was to investigate immune responses directed against a set of microbial antigens in patients with oral lichen planus and in matched controls.
Venous blood was obtained, and the mononuclear cells were enriched by density gradient centrifugation. The proliferation of peripheral blood mononuclear cells was assessed, following stimulation with purified protein derivative (PPD), Candida albicans, phytohemagglutinin or when cells were left unstimulated, after three or six days of cell culture. The production of interleukin-1ß (IL-1ß), IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α), G-CSF, GM-CSF, MCP-1, MIP-ß was assessed in supernatants using the Bio-plex(®) assay and was complemented with ELISA for selected cytokines.
Patients with oral lichen planus demonstrated reduced proliferative responses against PPD (P < 0.05) and C. albicans (P < 0.05). The majority of investigated cytokines, including the pro-inflammatory, IFN-γ and TNF-α were expressed at reduced levels in PPD-stimulated supernatants from patients with oral lichen planus.
Collectively, the findings suggested that memory lymphocytes from patients with oral lichen planus (OLP) may have an impaired functional ability to react against certain recall antigens, as part of a generalized response, which may reflect immune regulatory processes. Further studies are needed to clarify the mechanisms of down-regulation in OLP pathogenesis and progression.
据报道，苔藓样糠疹（一种影响黏膜和皮肤的自身免疫性疾病）存在细胞免疫功能障碍。我们的目的是研究口腔苔藓样患者针对一组微生物抗原的免疫反应，并与匹配的对照组进行比较。
采集静脉血，通过密度梯度离心法富集单核细胞。在细胞培养 3 或 6 天后，经纯化蛋白衍生物（PPD）、白色念珠菌、植物血凝素刺激或未刺激时，评估外周血单核细胞的增殖情况。采用 Bio-plex（®）检测试剂盒检测上清液中白细胞介素-1β（IL-1β）、IL-2、IL-4、IL-5、IL-6、IL-10、IL-12、IL-13、IL-17、干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、G-CSF、GM-CSF、MCP-1、MIP-ß的产生情况，并通过 ELISA 对选定的细胞因子进行补充。
口腔苔藓样患者对 PPD（P < 0.05）和白色念珠菌（P < 0.05）的增殖反应降低。在 PPD 刺激的上清液中，大多数研究的细胞因子，包括促炎细胞因子 IFN-γ和 TNF-α，表达水平降低。
总的来说，这些发现表明，口腔苔藓样患者的记忆淋巴细胞可能对某些回忆抗原的反应功能受损，作为广义反应的一部分，这可能反映了免疫调节过程。需要进一步研究来阐明 OLP 发病机制和进展中下调的机制。
