新发现的海洋放线菌谱系的抗氧化活性。

Antioxidant activity of newly discovered lineage of marine actinobacteria.

机构信息

Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.

出版信息

Asian Pac J Trop Med. 2013 Apr 13;6(4):325-32. doi: 10.1016/S1995-7645(13)60065-6.

DOI:10.1016/S1995-7645(13)60065-6

PMID:23608337

Abstract

OBJECTIVE

To investigate the antioxidant activity of marine actinobacteria.

METHODS

The content of total phenolics, the level of antioxidant potential by DPPH radical scavenging activity, metal chelating activity, FRAP method, β carotene assay and NO scavenging activity in extract were determined.

RESULTS

In all the methods the extract exhibited good scavenging activity except NO scavenging activity. The IC(50) values of marine actinobacteria extract on DPPH radical were found to be 41.09 μg/mL. The zone of color retention was 12 mm in β-carotene bleaching assay. DNA protective efficiency of the extracts was also studied using UV-photolysed H(2)O(2)-driven oxidative damage to pBR322. HPLC analysis identified some of the major phenolic compounds in extracts, which might be responsible for the antioxidant potential and cyto-protection. It showed a 100% cytotoxic effect in brine shrimp lethality assay within 10 mins. The novel actinobacteria was identified as Streptomyces LK-3 (JF710608) through 16S rDNA Sequencing.

CONCLUSIONS

The results obtained suggest that the extracts bear anti-cancer metabolites and could be considered as a potential source for anti-cancer drug development.

摘要

目的

研究海洋放线菌的抗氧化活性。

方法

测定提取物中总酚含量、DPPH 自由基清除活性、金属螯合活性、FRAP 法、β-胡萝卜素测定法和 NO 清除活性的抗氧化潜力。

结果

除了对 NO 的清除活性外，该提取物在所有方法中均表现出良好的清除活性。海洋放线菌提取物对 DPPH 自由基的 IC50 值为 41.09μg/ml。β-胡萝卜素褪色试验中，颜色保留区为 12mm。还研究了提取物对 UV 光解 H2O2 驱动的 pBR322 氧化损伤的 DNA 保护效率。HPLC 分析鉴定了提取物中一些主要的酚类化合物，这些化合物可能是其具有抗氧化潜力和细胞保护作用的原因。在 10 分钟内，它在卤虫致死试验中表现出 100%的细胞毒性作用。通过 16S rDNA 测序，将新型放线菌鉴定为链霉菌 LK-3（JF710608）。