Robles Francisco E, Samineni Prathyush, Wilson Jesse W, Warren Warren S
Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.
Opt Express. 2013 Apr 22;21(8):9353-64. doi: 10.1364/OE.21.009353.
Pump-probe microscopy is an imaging technique that delivers molecular contrast of pigmented samples. Here, we introduce pump-probe nonlinear phase dispersion spectroscopy (PP-NLDS), a method that leverages pump-probe microscopy and spectral-domain interferometry to ascertain information from dispersive and resonant nonlinear effects. PP-NLDS extends the information content to four dimensions (phase, amplitude, wavelength, and pump-probe time-delay) that yield unique insight into a wider range of nonlinear interactions compared to conventional methods. This results in the ability to provide highly specific molecular contrast of pigmented and non-pigmented samples. A theoretical framework is described, and experimental results and simulations illustrate the potential of this method. Implications for biomedical imaging are discussed.
泵浦-探测显微镜术是一种能够提供色素样本分子对比度的成像技术。在此，我们介绍泵浦-探测非线性相位色散光谱法（PP-NLDS），该方法利用泵浦-探测显微镜术和光谱域干涉测量法，从色散和共振非线性效应中获取信息。与传统方法相比，PP-NLDS将信息内容扩展到四个维度（相位、幅度、波长和泵浦-探测时间延迟），从而能够更深入地了解更广泛的非线性相互作用。这使得该方法能够为色素和非色素样本提供高度特异性的分子对比度。文中描述了一个理论框架，实验结果和模拟演示了该方法的潜力。还讨论了其在生物医学成像中的应用前景。
