Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
Opt Lett. 2011 Dec 1;36(23):4665-7. doi: 10.1364/OL.36.004665.
Nonlinear phase dispersion spectroscopy is introduced as a means to retrieve wideband, high spectral resolution profiles of the wavelength-dependent real part of the refractive index. The method is based on detecting dispersion effects imparted to a light field with low coherence transmitted through a thin sample and detected interferometrically in the spectral domain. The same sampled signal is also processed to yield quantitative phase maps and spectral information regarding the total attenuation coefficient using spectral-domain phase microscopy and spectroscopic optical coherence tomography (SOCT), respectively. Proof-of-concept experiments using fluorescent and nonfluorescent polystyrene beads and another using a red blood cell demonstrate the ability of the method to quantify various absorptive/dispersive features. The increased sensitivity of this method, novel to our knowledge, is compared to intensity-based spectroscopy (e.g., SOCT), and potential applications are discussed.
本文介绍了一种非线性相位色散光谱法，用于获取宽带、高光谱分辨率的波长相关折射率实部分布轮廓。该方法基于检测低相干光通过薄样品传输时产生的色散效应，并在光谱域中进行干涉探测。通过对同一采样信号进行处理，可以分别使用光谱域相衬显微镜和光谱学光相干层析术（SOCT）得到定量相位图和关于总衰减系数的光谱信息。使用荧光和非荧光聚苯乙烯珠的实验证明了该方法量化各种吸收/色散特征的能力，另一个使用红细胞的实验也验证了该方法的可行性。与基于强度的光谱学（如 SOCT）相比，该方法具有新颖的高灵敏度，我们对其进行了比较，并讨论了潜在的应用。
