Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Cancer Res. 2010 Dec 1;70(23):9562-9. doi: 10.1158/0008-5472.CAN-10-1554. Epub 2010 Nov 23.
Sensitive assays for rapid quantitative analysis of histologic sections, resected tissue specimens, or in situ tissue are highly desired for early disease diagnosis. Stained histopathology is the gold standard but remains a subjective practice on processed tissue taking from hours to days. We describe a microscopy technique that obtains a sensitive and accurate color-coded image from intrinsic molecular markers. Spectrally reconstructed nonlinear interferometric vibrational imaging can differentiate cancer versus normal tissue sections with greater than 99% confidence interval in a preclinical rat breast cancer model and define cancer boundaries to ± 100 μm with greater than 99% confidence interval, using fresh unstained tissue sections imaged in less than 5 minutes. By optimizing optical sources and beam delivery, this technique can potentially enable real-time point-of-care optical molecular imaging and diagnosis.
对于快速定量分析组织切片、切除组织标本或原位组织的敏感检测方法，人们有着强烈的需求，因为这种方法可以用于早期疾病诊断。染色的组织病理学检查仍然是金标准，但它是一种对经过数小时至数天处理的组织进行的主观操作。我们描述了一种从内在分子标记物获得敏感和准确的彩色编码图像的显微镜技术。在临床前大鼠乳腺癌模型中，光谱重建非线性干涉振动成像可以区分癌症与正常组织切片，置信区间大于 99%，并使用新鲜未染色的组织切片在不到 5 分钟的时间内以大于 99%的置信区间定义癌症边界至±100μm。通过优化光学源和光束传输，该技术有可能实现实时现场光学分子成像和诊断。
