KAUST Global Collaborative Research, Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, People's Republic of China.
Mar Biotechnol (NY). 2013 Oct;15(5):552-8. doi: 10.1007/s10126-013-9502-7. Epub 2013 Apr 25.
Biofouling results in tremendous economic losses to maritime industries around the world. A recent global ban on the use of organotin compounds as antifouling agents has further raised demand for safe and effective antifouling compounds. In this study, 49 secondary metabolites, including diterpenoids, steroids, and polyketides, were isolated from soft corals, gorgonians, brown algae, and fungi collected along the coast of China, and their antifouling activity was tested against cyprids of the barnacle Balanus (Amphibalanus) amphitrite. Twenty of the compounds were found to inhibit larval settlement significantly at a concentration of 25 μg ml(-1). Two briarane diterpenoids, juncin O (2) and juncenolide H (3), were the most promising non-toxic antilarval settlement candidates, with EC50 values less than 0.13 μg ml(-1) and a safety ratio (LC50/EC50) higher than 400. A preliminary structure-activity relationships study indicated that both furanon and furan moieties are important for antifouling activity. Intriguingly, the presence of hydroxyls enhanced their antisettlement activity.
生物污损给全球的航海工业带来了巨大的经济损失。最近，全球范围内禁止使用有机锡化合物作为防污剂，这进一步增加了对安全有效的防污化合物的需求。在这项研究中，从中国沿海采集的软珊瑚、柳珊瑚、褐藻和真菌中分离出了 49 种次生代谢产物，包括二萜、甾体和聚酮类化合物，并测试了它们对藤壶幼虫（Balanus (Amphibalanus) amphitrite）的防污活性。在 25μg/ml 的浓度下，有 20 种化合物显著抑制了幼虫的附着。两种布瑞安烷二萜，juncin O（2）和 juncenolide H（3）是最有前途的无毒抗幼虫附着候选物，EC50 值小于 0.13μg/ml，安全比（LC50/EC50）大于 400。初步的结构-活性关系研究表明，呋喃酮和呋喃部分对于防污活性都很重要。有趣的是，羟基的存在增强了它们的抗附着活性。
