Janbaz Khalid Hussain, Jan Asma, Qadir M Imran, Gilani Anwarul Hassan
Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
Acta Pol Pharm. 2013 Mar-Apr;70(2):261-9.
The methanolic extract of the whole plant of Tephrosia pupurea, Linn. was subjected to find out its possible therapeutic utility to validate its folkloric use in native systems of medicine. The extract on application to spontaneous contractions in isolated rabbit jejunum preparations exerted a concentration dependent (0.003-3.0 mg/mL) relaxant effect. The extract also caused concentration dependent relaxation of K+(80 mM)-induced spastic contractions. These findings were further supported by the observations that the extract caused a concentration dependent right ward shift of the Ca2+ response curves in manner similar to that of verapamil. The extract exhibited a relaxant effect on carbachol and high K+ (80 mM)-induced contractions of isolated rabbit tracheal preparations in a manner similar to verapamil. The observed non-specific bronchodilator response is possibly mediated through Ca2+ channel blockade. Moreover, the extract also exhibited a dose dependent relaxant effect on phenylephrine (1 microM) and K+(80 mM)-induced contractions in a manner similar to verapamil. On the basis of the above-mentioned findings, it can be concluded that the use of Tephrosia purpurea, in gastrointestinal spasm, asthma and hypertension is likely to be mediated through calcium channel blockage.
对紫穗槐（Tephrosia pupurea, Linn.）全株的甲醇提取物进行研究，以确定其可能的治疗效用，从而验证其在传统医学体系中的民间药用价值。将该提取物应用于离体兔空肠标本的自发收缩时，呈现出浓度依赖性（0.003 - 3.0 mg/mL）的松弛作用。该提取物还能引起K⁺（80 mM）诱导的痉挛性收缩的浓度依赖性松弛。提取物使Ca²⁺反应曲线呈浓度依赖性向右移动，其方式与维拉帕米相似，这一观察结果进一步支持了上述发现。提取物对卡巴胆碱和高K⁺（80 mM）诱导的离体兔气管标本收缩表现出松弛作用，其方式与维拉帕米相似。观察到的非特异性支气管扩张反应可能是通过Ca²⁺通道阻滞介导的。此外，提取物对去氧肾上腺素（1 microM）和K⁺（80 mM）诱导的收缩也表现出剂量依赖性松弛作用，其方式与维拉帕米相似。基于上述发现，可以得出结论，紫穗槐在治疗胃肠痉挛、哮喘和高血压方面的作用可能是通过钙通道阻滞介导的。
