Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
J Ethnopharmacol. 2021 Mar 1;267:113634. doi: 10.1016/j.jep.2020.113634. Epub 2020 Nov 25.
The conventional naturopaths of Punjab Province (Pakistan) have trivial usage of Anagallis arvensis Linn.(Primulaceae) for cure of diarrhea, constipation, asthma as well as hypertension.
Present research was focused to discover comprehensive mechanism of spasmogenic, spasmolytic, bronchorelaxant and hypotensive folkloric usage of Anagallis arvensis Linn..
The crude extract of Anagallis arvensis Linn. (Aa.Cr) & its (aqueous & organic) portions tested in-vitro on isolated jejunum, ileum, trachea, aorta, paired atria preparations as well as in-vivo in mice & normotensive anaesthetized rats. The responses have been noted by transducers (isotonic & isometric) coupled to Power Lab.
Anagallis arvensis Linn. (Aa.Cr; crude aqueous-alcoholic extract) produced contractile action at low concentrations but relaxant action was observed by increasing concentrations on spontaneous contractions of isolated jejunum of rabbit. But, pre-treatment of tissue with atropine prior extract caused suppression of contractile effect indicating presence of cholinergic muscarinic response of Aa.Cr. It also triggered relaxation of high Potassium -stimulated contractions of jejunum with subsequent non-parallel right move in Ca CRCs. Moreover, Aa.Cr relaxed carbachol - & high Potassium - stimulated contractions in trachea of rabbit but observed relaxant effect was powerful against CCh (1 μM)- stimulated contractions with rightside parallel move of CCh-curves succeeded by non-parallel move, like Dicyclomine, having dual activities. The Aa.Cr also showed relaxant result on Phenylephrine and High Potassium -prompted contractions in endothelium intact aorta. The fractionation revealed segregations of contractile & relaxant effects in relevant aqueous & organic portions. The Intravenous administration of Aa.Cr to ketamine-diazepam anaesthetized normo-tensive albino rats resulted in decreased MABP, SBP & DBP. The Aa.Cr applied negative (-) inotropic & chronotropic action on paired atria. The Aa.Cr also exhibited anti-diarrheal action in mice against castor oil prompted diarrhea and also mitigated distance covered by charcoal meal in gastrointestinal tract in a manner comparable with loperamide.
These results revealed presence of CCB and selective muscarinic agonist activity in Aa.Cr, hence validating folkloric practice of Anagallis arvensis Linn. in diarrhea, constipation, asthma & hypertension.
旁遮普省（巴基斯坦）的传统自然疗法医生将马齿苋（报春花科）用于治疗腹泻、便秘、哮喘和高血压。
本研究旨在探索马齿苋的痉挛、松弛、支气管扩张和降压的民间用途的综合机制。
将马齿苋的粗提取物（Aa.Cr）及其（水和有机）部分在体外作用于分离的空肠、回肠、气管、主动脉、成对心房制剂，以及体内作用于小鼠和正常麻醉大鼠。通过与 Power Lab 耦合的换能器（等张和等容）记录响应。
马齿苋（Aa.Cr；粗水醇提取物）在低浓度下产生收缩作用，但在增加浓度时观察到对兔离体空肠自发性收缩的松弛作用。但是，在用提取物预处理组织之前，用阿托品处理会抑制收缩作用，表明 Aa.Cr 存在胆碱能毒蕈碱 M 受体反应。它还触发了高钾刺激的空肠收缩的松弛，随后在 Ca CRCs 中出现非平行的右移。此外，Aa.Cr 松弛了兔气管中乙酰胆碱和高钾刺激的收缩，但观察到的松弛作用对 CCh（1 μM）刺激的收缩更有效，CCh 曲线随后出现右侧平行移动，类似于双环胺，具有双重活性。Aa.Cr 还对内皮完整的主动脉中苯肾上腺素和高钾引起的收缩表现出松弛作用。分馏揭示了相关水相和有机部分中的收缩和松弛作用的分离。静脉给予 Aa.Cr 给氯胺酮-地西泮麻醉的正常血压白化大鼠导致平均动脉压、收缩压和舒张压降低。Aa.Cr 对配对心房表现出负性（-）变力和变时作用。Aa.Cr 还在小鼠中表现出抗腹泻作用，对抗蓖麻油引起的腹泻，并以类似于洛哌丁胺的方式减轻胃肠道中炭粉覆盖的距离。
这些结果表明 Aa.Cr 中存在 CCB 和选择性毒蕈碱激动剂活性，因此验证了马齿苋在腹泻、便秘、哮喘和高血压中的民间应用。
