Aslam Naveed, Janbaz Khalid Hussain
a Department of Pharmacy , Bahauddin Zakariya University , Multan , Pakistan.
b Akson College of Pharmacy , MUST , Mirpur , Azad Kashmir , Pakistan.
Pharm Biol. 2017 Dec;55(1):1131-1137. doi: 10.1080/13880209.2017.1291691.
Salsola imbricata Forssk. (Chenopodiaceae) has folkloric repute for the treatment of various gastrointestinal and respiratory ailments.
The present study investigates spasmolytic and bronchorelaxant effects of S. imbricata.
The crude aqueous-ethanol extract of the aerial parts of S. imbricata and its fractions, in cumulative concentrations (0.01-10 mg/mL), were tested on contractions of isolated rabbit jejunum and tracheal preparations. Furthermore, concentration response curves (CRCs) of Ca and carbachol were constructed in the absence and presence of the extract. Standard organ bath methods were used.
The crude extract relaxed spontaneous, K(80 mM) and carbachol (1 μM)-induced contractions in jejunum preparations with respective EC values of 0.40 (0.35-0.46), 0.69 (0.60-0.79) and 0.66 (0.57-0.75) mg/mL. It shifted Ca CRCs rightward in nonparallel manner. In isolated tracheal preparations, the crude extract caused relaxation of K(80 mM) and carbachol (1 μM)-induced contractions with EC values of 0.86 (0.75-0.98) and 0.74 (0.66-0.84) mg/mL, respectively. It displaced carbachol CRCs rightward with suppression of maximal response. In both tissues, pretreatment with propranolol (1 μM) caused rightward shift in inhibitory CRCs of the extract against carbachol-induced contractions. The ethyl acetate fraction was found more potent in relaxing smooth muscle contractions than the parent extract and its aqueous fraction.
The results suggest that the spasmolytic and bronchorelaxant activities of S. imbricata are related to Ca antagonistic and β-adrenergic agonistic effects, thus justifying some of the traditional uses of the plant.
叉毛蓬（藜科）在民间被认为可用于治疗各种胃肠道和呼吸道疾病。
本研究考察叉毛蓬的解痉和支气管舒张作用。
采用累积浓度（0.01 - 10mg/mL）的叉毛蓬地上部分粗水乙醇提取物及其馏分，对离体兔空肠和气管标本的收缩进行测试。此外，在有和无提取物存在的情况下构建钙和卡巴胆碱的浓度 - 反应曲线（CRC）。使用标准的器官浴方法。
粗提取物可舒张空肠标本中自发的、K（80mM）和卡巴胆碱（1μM）诱导的收缩，其相应的半数有效浓度（EC）值分别为0.40（0.35 - 0.46）、0.69（0.60 - 0.79）和0.66（0.57 - 0.75）mg/mL。它使钙CRC呈非平行向右移位。在离体气管标本中，粗提取物可舒张K（80mM）和卡巴胆碱（1μM）诱导的收缩，EC值分别为0.86（0.75 - 0.98）和0.74（0.66 - 0.84）mg/mL。它使卡巴胆碱CRC向右移位并抑制最大反应。在两种组织中，用普萘洛尔（1μM）预处理可使提取物对卡巴胆碱诱导收缩的抑制CRC向右移位。发现乙酸乙酯馏分在舒张平滑肌收缩方面比母体提取物及其水馏分更有效。
结果表明，叉毛蓬的解痉和支气管舒张活性与钙拮抗和β - 肾上腺素能激动作用有关，从而证明了该植物的一些传统用途。
