Lin Zhixiong, Khong Brian, Kwok Shirley, Cao Hongbin, West Robert B, Le Quynh-Thu, Kong Christina S
Department of Radiation Oncology, Shantou University Medical College, Shantou, China.
Head Neck. 2014 May;36(5):709-14. doi: 10.1002/hed.23362. Epub 2013 Oct 26.
We evaluated the relationship of human papillomavirus (HPV) and Epstein-Barr virus (EBV) with race in endemic and nonendemic cohorts of patients with nasopharyngeal carcinoma (NPC), and with smoking status in the nonendemic cohort.
Tissue microarrays (TMAs) were constructed using samples from 86 patients treated in southern China and 108 patients from Stanford, California. TMAs were stained with p16, HPV in situ hybridization (ISH), and EBV ISH. Polymerase chain reaction (PCR) was used to confirm EBV(-) cases and HPV status in p16(+) cases. Survival data was available for the Stanford cohort only.
No HPV(+) cases were detected in the Chinese cohort. In the Stanford cohort, 5 of 11 EBV(-) cases harbored HPV-16, 10 of 10 occurred in whites, and 8 of 11 were smokers. Patients with EBV(-) NPC also showed a trend toward worse survival.
EBV(-) NPC shows an association with the presence of HPV, white race, and smoking. In contrast, EBV(-) NPC shows no association with HPV in the endemic cohort.
我们评估了人乳头瘤病毒（HPV）和爱泼斯坦-巴尔病毒（EBV）与鼻咽癌（NPC）的地方性和非地方性队列患者种族的关系，以及与非地方性队列患者吸烟状况的关系。
使用来自中国南方接受治疗的86例患者和加利福尼亚州斯坦福大学的108例患者的样本构建组织微阵列（TMA）。TMA用p16、HPV原位杂交（ISH）和EBV ISH染色。聚合酶链反应（PCR）用于确认EBV（-）病例和p16（+）病例中的HPV状态。仅斯坦福队列有生存数据。
中国队列中未检测到HPV（+）病例。在斯坦福队列中，11例EBV（-）病例中有5例携带HPV-16，10例中有10例发生在白人中，11例中有8例是吸烟者。EBV（-）NPC患者也显示出生存较差的趋势。
EBV（-）NPC与HPV的存在、白人种族和吸烟有关。相比之下，在地方性队列中，EBV（-）NPC与HPV无关。
